HCA056A detects glutathione-S-transferase (GST), a family of cytosolic dimeric isoenzymes that play an important role in detoxification by catalyzing the conjugation of reduced glutathione to a myriad of hydrophobic and electrophilic compounds. GSTs may also bind toxins and function as transport proteins.
Mammalian GSTs are 45–55kDa in size and are assigned to at least four generic classes: Alpha, Mu , Pi and Theta. The amino acid sequence GST is highly conserved in most organisms, including mammals.
GST is a commonly used tag in protein expression systems.
Product Form
A lyophilised bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using metal chelate affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a DYKDDDDK-tag and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
Western Blotting
1/20 - 1/200
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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