HCA035A recognises beta-galactosidase, an enzyme that acts to catalyse the hydrolysis of beta-galactosides into monosaccharides.
Beta-galactosidase is a homo-tetrameric protein, containing four identical sub-units of approximately 116kD each. The enzyme is commonly used as a reporter gene in molecular biology to determine the success of transfections.
Competitive binding studies demonstrate that HCA035A binds to an epitope distinct fromHCA034, but to an epitope identical to, or structurally adjacent toHCA033.
Product Form
A monovalent human recombinant Fab selected from the HuCAL®GOLD phage display library. Expressed inE. coliand purified using NiNTA affinity chromatography. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
1/2000 - 1/50000
Immunohistology - Frozen
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Shelf Life
6 months from date of reconstitution.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
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