Anti mouse farnesyl diphosphate synthetase antibodyrecognizes the C-terminal 116 amino acids of farnesyl diphosphate synthetase.
Due to high homology, the antibody may cross-react with the farnesyl diphosphate synthetase of other species. The cross-species identity is: rat 98%, bovine 89%, human 89%, orang utan 89%, chimpanzee 89%, chicken 86%.
The mKIAA1293 protein, also known as farnesyl diphosphate synthetase, catalyzes the formation of a key cellular intermediate in isoprenoid metabolic pathways. The sequential condensation of isopentenyl pyrophosphate with the allylic pyrophosphate, dimethylallyl pyrophosphate, and then with the resultant geranylpyrophosphate is catalyzed to ultimately yield the product farnesyl pyro-phosphate. Beside the isoprene and cholesterol biosynthesis, the enzyme seems to be active in the fibroblast growth factor (FGF)-mediated signalling as a modulator of the cellular response to FGF treatment. The cytoplasmic enzyme forms a homodimer and belongs to the FPP/GGPP synthetase family.
Product Form
A lyophilised bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
ELISA
2ug/ml
Immunohistology - Frozen
6.8ug/ml
Immunohistology - Paraffin
Immunoprecipitation
Western Blotting
0.5ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
6 months from date of reconstitution.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European patent 0,859,841 and corresponding patents.
This antibody was developed by AbD Serotec, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany, in collaboration with the Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818 JAPAN.
WB data kindly provided by the Kazusa DNA Research Institute, Kisarazu, Japan.
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