HCA007 recognises the mFLJ00279 protein, also known as non-muscle myosin heavy chain type A, a critical component of the cellular contractile apparatus. It is involved in maintaining cell-cell adhesion in early mammalian embryos and ablation of this protein leads to prenatal lethality. Mutation in the human MYH9 gene lead to MYH9-related hereditary macrothrombocytopenia (HMTC) characterized by a reduced number of blood platelets.
Species Cross-Reactivity
|
Target Species |
Cross Reactivity |
|
Bovine |
Expected from Sequence |
|
Chicken |
Expected from Sequence |
|
Dog |
Expected from Sequence |
|
Human |
Expected from Sequence |
|
Rat |
Expected from Sequence |
Product Form
A lyophilised bivalent human recombinant Fab selected from the HuCAL® GOLD phage display library. Expressed in E. coli and purified using NiNTA affinity chromatography. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain.
Activity
Activity was tested by indirect ELISA: recombinant purified mFLJ00279 (5 µg/ml) plus unrelated control proteins were immobilized on a microtiter plate. Specific binding was monitored by first adding HCA007 (2 µg/ml), then adding a secondary antibody (goat anti-human F(ab')2 fragment specific, AP conjugate. 1:5000 diluted). A fluorescent signal was created by adding the AP substrate Attophos. The signal on the antigen is at least 5-fold above background, whereas the signal on the control antigens is less than 1.5-fold above background.
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit
www.abdserotec.com/protocols.
|
Application Name |
Yes |
No |
Not Determined |
Suggested Dilution |
|
ELISA |
|
|
|
2ug/ml |
|
Immunohistology - Frozen |
|
|
|
2ug/ml |
|
Immunohistology - Paraffin |
|
|
|
|
|
Western Blotting |
|
|
|
2ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
HCA007 detects a band of approximately 210 kDa in mouse lung cell lysates .
Technical Advice
Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of reconstitution.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by AbD Serotec.
ELISA
Due to the presence of bovine serum albumin (BSA), this antibody is unsuitable for use as a capture reagent in sandwich ELISA applications. This product is also available without BSA,
please enquire.
UniProt
Health And Safety Information
Acknowledgements
Sold under license of U.S. Patents 6,300,064, 6,696,248, 6,708,484, 6,753,136, European Patent 0,859,841 and corresponding patents.
This antibody was developed by
AbD Serotec, a wholly owned subsidiary of Bio-Rad Laboratories Inc., Zeppelinstr. 4, 82178 Puchheim, Germany, in collaboration with the
Kazusa DNA Research Institute, 2-6-7 Kazusa-kamatari, Kisarazu, Chiba 292-0818 JAPAN.
WB data kindly provided by the Kazusa DNA Research Institute, Japan.
Regulatory
For research purposes only
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