Optimization is essential for any RNAi screen. The Thermo Scientific ON-TARGETplus Optimization Plates are ideal for selection of the best transfection conditions and controls for screens in human cells using RTF siRNA Libraries. The plate layout enables the testing of cell density and transfection reagent amount on the same plate while also identifying the best non-targeting siRNA control for your assay.
Highlights
• Three ready-to-use replicate 96-well plates; 6.25 pmol siRNA per well (50 nM final concentration)
• Provided in clear or clear-bottomed white or black-walled plates to support assays involving fluorescent or luminescent detection
• Positive control pool targets Human cyclophilin B (Accession NM_000942)
• Four non-targeting siRNAs and a non-targeting pool with ON-TARGETplus modifications to minimize off-target effects
Row
Pre-plated control
Control Cat No.
A
ON-TARGETplus Non-targeting siRNA #1
D-001810-01
B
ON-TARGETplus Non-targeting siRNA #2
D-001810-02
C
ON-TARGETplus Non-targeting siRNA #3
D-001810-03
D
ON-TARGETplus Non-targeting siRNA #4
D-001810-04
E
ON-TARGETplus Non-targeting pool
D-001810-10
F
ON-TARGETplus Human Cyclophilin B control pool
D-001820-10
For a diagram of the RTF Library Plate layout, see Figure 1 on the Supporting Data tab.
See Figure 1 (Supporting data tab) for the Optimization Plate layout.
DharmaFECT Cell Culture Reagent (DCCR) is recommended for use with RTF libraries to dilute transfection reagents prior to use. DCCR may be purchased separately or accompanying your RTF Optimization Plates
DharmaFECT transfection reagents and are highly recommended for use with RTF libraries, and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
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