Optimization is essential for any RNAi screen. The Thermo Scientific siGENOME Optimization Plates for Human or Mouse are ideal for selection of the best transfection conditions and controls for screens in human or mouse cells using RTF siRNA Libraries. The plate layout enables the testing of cell density and transfection reagent amount on the same plate while also identifying the best non-targeting siRNA control for your assay.
Highlights
• Three ready-to-use replicate 96-well plates; 6.25 pmol siRNA per well (50 nM final concentration)
• Provided in clear or clear-bottomed white- or black-walled plates to support assays involving fluorescent or luminescent detection
• Positive control pool targets cyclophilin B in Human (NM_000942), Mouse (NM_011149), and Rat (NM_022536)
• Four non-targeting siRNAs and a non-targeting pool designed to have at least 4 mismatches to known human, mouse, and rat genes
Row
Pre-plated control
Control Catalog No.
A
siGENOME Non-targeting siRNA #2
D-001210-02
B
siGENOME Non-targeting siRNA #3
D-001210-03
C
siGENOME Non-targeting siRNA #4
D-001210-04
D
siGENOME Non-targeting siRNA #5
D-001210-05
E
siGENOME Non-targeting pool #2
D-001206-14
F
siGENOME Cyclophilin B control pool (H, M, R) Control siRNA
D-001136-01
See Figure 1 (Supporting data tab) for the Optimization Plate layout.
Experimental considerations
DharmaFECT Cell Culture Reagent (DCCR) is recommended for use with RTF libraries to dilute transfection reagents prior to use. DCCR may be purchased separately or accompanying your RTF Optimization Plates
DharmaFECT transfection reagents are highly recommended for use with RTF libraries and should be purchased separately. Refer to the DharmaFECT Cell Type Guide to find the appropriate formulation for your cell type.
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