CellTiter-Glo? 3D Cell Viability Assay

产品编号：G9681 品牌：promega 原厂货号：G9681
产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞活性及活力检测试剂盒
应用分类：

包装：

10ml

运保温度:

–30 到 –10°C

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其它组分：

CellTiter-Glo® 3D Reagent【子货号：G968A，包装：1 x 10ml，，运保温度：–30 到 –10°C】

描述：

说明

CellTiter-Glo® 3D Cell Viability Assay （ CellTiter-Glo® 3D 发光法细胞活力检测试剂盒）通过对 ATP 的定量来测定 3D 培养细胞的细胞活力。 ATP 是活细胞新陈代谢的指标。试剂盒提供了即用型的检测试剂，实验前无需预先将冻干底物与反应缓冲液混合，使用方便。 CellTiter-Glo® 3D 细胞活力检测试剂的特殊配方使其拥有更强劲的裂解能力，专门用于检测 3D 细胞培养产生的微组织（ microtissue ）的细胞活力。无需细胞洗涤、去除培养基或多步移液操作，可直接使用单一 CellTiter-Glo® 3D 检测试剂进行均质检测，兼容血清；可应用于多种规格多孔板，适用于高通量筛选（ HTS ）。 CellTiter-Glo® 3D 检测试剂盒经验证已成功应用于多种方式培养的 3D 微组织的活力检测，包括细胞悬滴培养板（ hanging-drop plate ）、超低吸附细胞培养板、 Matrigel™ 包被的细胞培养板、琼脂糖包被细胞板、甲基纤维素覆盖培养液和 Alvetex® 三维细胞培养体系中培养的 3D 微组织。

特点

• 性能强劲，可渗入微组织： 和其他细胞活力检测方法相比，拥有更强的裂解能力，使得试剂盒可检测种类广泛的微组织

•即用型试剂： 无需试剂混合操作；仅需简单的“加样 - 混合 - 检测”操作；方便用于高通量筛选。

•快速获得结果： 相比比色或荧光法细胞活力检测方法速度更快，可在 30 分钟内获得结果。

•卓越的灵敏度： 相比比色或荧光法细胞活力检测，拥有更高的信噪比。

•灵活： 可用于多种多孔板检测格式（ 96- 孔及常规 / 小 - 体积 384- 孔板）；数据可使用发光检测仪， CCD 或其他可读取多孔板发光信号的仪器收集。

•辉光型信号： 发光信号稳定，半衰期 >3 小时（与细胞类型和培养基有关）；可用于批量或连续检测。

应用

3D 细胞培养微组织或常规培养细胞的细胞活力和细胞毒性检测。

原厂资料：

The CellTiter-Glo® 3D Cell Viability Assay is a homogeneous method to determine the number of viable cells in 3D cell culture based on quantitation of the ATP present, which is a marker for the presence of metabolically active cells. This ready-to-use reagent is based on the original CellTiter-Glo® Luminescent Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when preparing reagent. The CellTiter-Glo® 3D Cell Viability Assay is formulated with more robust lytic capacity and is designed for use with microtissues produced in 3D cell culture. The homogeneous assay procedure involves addition of a single reagent (CellTiter-Glo® 3D Reagent) directly to cells cultured in serum-supplemented medium. Cell washing, removal of medium and multiple pipetting steps are not required. This assay is compatible with multiwell-plate formats, making it ideal for automated high-throughput screening (HTS). The CellTiter-Glo® 3D Assay has been used successfully with 3D microtissue cell culture produced via hanging-drop plates, ultra-low attachment plates, Matrigel®-coated plates, agarose-coated plates, cultures suspended in methylcellulose and Alvetex® plates.

Features - Benefits

Robust Penetration into Microtissues: Improved lytic capacity allows use over a broad range of microtissue sizes compared to other viability assay methods.
Ready-to-Use Reagent: No mixing of components required; simply thaw, equilibrate to room temperature and "add-mix-incubate-measure". Convenient for HTS applications.
Fast Results: Data can be recorded in 30 minutes or less after adding reagent, quicker than when using colorimetric or fluorometric viability assays.
Superior Sensitivity: The signal-to-background ratio of this assay applied to microtissues is much greater than that of standard colorimetric and fluorometric assays.
Flexible Format: The assay can be used with various multiwell formats (96-well and regular or low-volume 384-well). Data can be recorded by luminometer, CCD camera or other imaging devices capable of reading luminescence in multiwell plates.
Glow-Type Signal: Stable luminescent signal half-life >3 hours, depending on cell type and culture medium used, allows batch mode or consecutive processing of multiple plates.

Applications

Cell viability assays with 3D microtissue cell culture or standard cell culture samples.
Cytotoxicity assays with 3D microtissue cell culture or standard cell culture samples.