HaloCHIP? System

• 产品编号：G9410      品牌：promega       原厂货号：G9410
• 产品分类：蛋白质检测与分析 > 蛋白质相互作用分析 > 免疫共沉淀
• 应用分类：

其它组分：

描述：

原厂资料：

The HaloCHIP™ System is a novel method designed for the covalent capture of intracellular protein:DNA complexes without the use of antibodies and offers an efficient and robust alternative to the standard chromatin immunoprecipitation (ChIP) method. Proteins of interest are expressed in cells as HaloTag® fusion proteins, crosslinked to DNA with formaldehyde and then captured on HaloLink™ Resin, which forms a highly specific, covalent interaction with the HaloTag® portion of the fusion protein. Stringent washing removes nonspecific proteins and DNA, and heating reverses the crosslinks between the DNA and the fusion protein and releases the captured DNA fragment, which subsequently can be purified.

For more information about the HaloTag® Flexi® Vectors, please visit the HaloTag® Technology Products page.

Features - Benefits

• No Requirement for Antibody: No need to make your own or purchase expensive, qualified antibodies.
• Obtain Results Faster: Obtain data in 24–48 hours with fewer steps to minimize potential experimental errors.
• Improved Signal-to-Noise Ratios: Enables detection of small changes in protein binding patterns using a minimal number of cells.

Applications

• Protein:DNA interaction analysis.