MultiTox-Fluor Multiplex Cytotoxicity Assay

产品编号：G9201 品牌：promega 原厂货号：G9201
产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞毒性检测试剂盒
应用分类：

包装：

5 ×10ml

运保温度:

-20℃

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其它组分：

GF-AFC Substrate【子货号：G608A，包装：5 x 10μl，，运保温度：-20℃】

bis-AAF-R110 Substrate【子货号：G609A，包装：5 x 10μl，，运保温度：-20℃】

Assay Buffer【子货号：G610A，包装：5 x 10ml，，运保温度：-20℃】

描述：

说明

MultiTox-Fluor Multiplex Cytotoxicity Assay(MultiTox-Fluor 叠加细胞毒性检测系统) 是一种均质的单溶液荧光检测试剂，可同时检测细胞培养孔中活细胞和死细胞的数量。此试剂盒通过测定两种不同的蛋白酶活性，实现了同时检测细胞活力和细胞毒性。活细胞蛋白酶的活性仅在完整的活细胞中才可以表现出来，是通过一种可穿透细胞膜的荧光肽类底物(GF-AFC) 来实现检测的。当细胞膜完整性丧失后，这种活细胞的蛋白酶标志物泄漏到周围培养基中，随即失活。该系统还含有另一种荧光肽类底物(bis-AAF-R110)，这种底物不能穿透细胞膜，用于检测死细胞蛋白酶活性，这种酶会从已经丧失膜完整性的细胞中释放出来。

特点

• 同时检测培养基中活细胞和死细胞的数量：一种均质检测的单溶液试剂，只需" 加样- 混合- 检测"。

• 细胞数数据归一化：活细胞和死细胞比值不依赖于细胞数量和归一化数据。细胞数数据的归一化使孔与孔、板与板、以及不同时间的检测结果更具可比性。

• 减少假阳性和假阴性数据：活细胞数和死细胞数互补，独立的检测成分互为内对照。

• 每个孔中能够得到更多数据：能与Promega 大部分基于生物发光的细胞凋亡或报告基因试剂盒叠加使用。

• 减少了测量变异：均质检测，" 加样- 混合- 检测" 的检测步骤避免了由于叠加操作步骤而出现的累加误差。

原厂资料：

The MultiTox-Fluor Multiplex Cytotoxicity Assay is a single-reagent-addition, homogeneous, fluorescent assay that measures the number of live and dead cells simultaneously in culture wells. The assay simultaneously measures cell viability and cytotoxicity by detecting two distinct protease activities. The live-cell protease activity is restricted to intact viable cells and is measured using a fluorogenic, cell-permeant peptide substrate (GF-AFC Substrate). The substrate enters intact cells where it is cleaved to generate a fluorescent signal proportional to the number of living cells. This live-cell protease activity marker becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium. A second, cell-impermeant, fluorogenic peptide substrate (bis-AAF-R110 Substrate) is used to measure dead-cell protease activity that has been released from cells that have lost membrane integrity.

Features - Benefits

Measure the Number of Live and Dead Cells in Culture: Homogeneous, "add-mix-measure" protocol eliminates parallel plate processing and reduces cell culture costs.
Normalize Data for Cell Number: The ratio of live:dead cells is independent of cell number and normalizes data. Data normalization for cell number makes results more comparable well-to-well, plate-to-plate, day-to-day.
Reduce False-Positive and -Negative Results: Complementary live- and dead-cell measures with independent chemistries serve as internal controls for each other.
Get More Data from Every Well: Multiplex the MultiTox-Fluor Assay with most Promega bioluminescent cell-based apoptosis or genetic reporter assays.
Reduce Assay Variability: The homogeneous "add-mix-measure" protocol avoids the cumulative error associated with multistep protocols.

Applications

Determine viability, cytotoxicity or both in the same well.
Multiplex with Promega luminescent cell-based assays.

Notes

G9200 contains sufficient reagents for 100 assays at 100μl/assay in a 96-well format or 400 assays at 25μl/assay in a 384-well format. G9201 contains sufficient reagents for 500 assays at 100μl/assay in a 96-well format or 2,000 assays at 25μl/assay in a 384-well format. G9202 contains sufficient reagents for 1,000 assays at 100μl/assay in a 96-well format or 4,000 assays at 25μl/assay in a 384-well format.