NAD(P)H-Glo? Detection System

• 产品编号：G9062      品牌：promega       原厂货号：G9062
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞代谢检测试剂盒
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描述：

原厂资料：

The in vitro enzyme-based NAD(P)H-Glo™ Detection System is a homogeneous, bioluminescent assay that quantitatively monitors the concentration of the reduced forms of nicotinamide adenine dinucleotides, NADH and NADPH, and does not discriminate between them. The oxidized forms, NAD+ and NADP+, are not detected and do not interfere with quantitation.

In the presence of NAD(P)H, a reductase enzyme reduces a proluciferin reductase substrate to form luciferin. Luciferin then is quantified using Ultra-Glo™ Recombinant Luciferase, and the light signal produced is proportional to the amount of NAD(P)H in the sample. The reductase and luciferase reactions are initiated by adding an equal volume of a single reagent, which contains reductase, proluciferin Reductase Substrate and Ultra-Glo™ Recombinant Luciferase, to a NAD(P)H-containing sample.

The assay is rapid, requiring only a 40- to 60-minute incubation, has a broad linear range and high signal-to background ratio. The assay is well suited to measuring NAD(P)H production or consumption in high-throughput formats.

Features - Benefits

Broad Linear Range: The NAD(P)H-Glo™ Detection System detects 0.1μM to 25μM NAD(P)H.
High Sensitivity: The limit of detection is ≤0.1μM NADH, with a maximum assay window (i.e., signal-to-background ratio) of 250. The system detects 1μM with a signal higher than fivefold over background.
Automation Compatible: The add-and-read format is compatible with automated and high-throughput protocols. Reactions are scalable and can be performed at low volumes in 96-, 384- and 1536-well plates.
Reliability and Reproducibility: The NAD(P)H-Glo™ Detection System routinely yields Z´ factors >0.7.
Stable Signal: The glow-type signal is stable, with a half-life greater than two hours, allowing batch plate processing.
Luminescence-Based NAD(P)H Detection: The luminescent format avoids fluorescent interference due to reagents and test compounds sometimes seen in fluorescent assays.
Applications

Monitoring the activity of enzymes that produce or use NAD(P)H.
Measuring NAD(P)H production or consumption in high-throughput formats.

注意事项：

G9061 contains sufficient reagents to perform 200 reactions in 96-well plates (50μl of sample + 50μl of NAD(P)H-Glo™ Detection Reagent), 400 assays in 384-well plates (25μl of sample + 25μl of NAD(P)H-Glo™ Detection Reagent) or 2,000 assays in low-volume 384-well plates (5μl of sample + 5μl of NAD(P)H-Glo™ Detection Reagent). Assay volumes can be varied depending on plate format as long as a 1:1 ratio of sample to NAD(P)H-Glo™ Detection Reagent is maintained.