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Viral ToxGlo? Assay

Viral ToxGlo? Assay

产品编号：G8941 品牌：promega 原厂货号：G8941
产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞活性及活力检测试剂盒
应用分类：

包装：

10ml

运保温度:

–20°C

到货周期：

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其它组分：

ATP Detection Buffer【子货号：G806A，包装：1 x 10ml，，运保温度： –20°C】

ATP Detection Substrate【子货号：V363A，包装：1 x 1 vial，，运保温度： –20°C】

描述：

说明

Viral ToxGlo™ Assay 是一种均质、简单、定量的测定裂解性病毒粒所引起的宿主细胞产生致细胞病变效应（ cytopathic effect ， CPE ）的方法。该方法将 ATP 作为宿主细胞活力指标， ATP 含量与存活的宿主细胞数成正比。病毒感染引起 CPE ，测定 ATP 的减少，可以反映病毒含量，定量测定病毒引起的致细胞病变效应。这种均质的“加样 - 混合 - 检测”方法，在病毒处理后，仅需加入单一试剂（ ATP 检测试剂）到宿主细胞即可完成操作，产生的“辉光型”信号与 ATP 含量成正比，无需洗涤细胞、多步移液操作及人工计数等繁琐操作。加样混合后， 10 分钟即可进行检测，在 384 孔格式下可检测到低至 15 个细胞 / 孔，该系统快速、灵敏，非常适合用于自动化的高通量筛选（ HTS ）。

特点

• 客观定量致细胞病变效应： 发光发检测提供定量结果，避免因操作者因视觉计数引起的主观错误。

• 超快速获得结果： 加入检测试剂后， 10 分钟即可进行检测，并获取实验结果。

• 简化致细胞病变效应的评估： 相比原有的 CPE 评估方法，均质的“加样 - 混合 – 检测”步骤，大大减少手动操作步骤。

• 规格可选： 可用于 96- 至 1536- 孔板格式检测。

• 适用于高通量筛选： 发光信号十分稳定，半衰期〉 5 小时（因所用培养基和细胞类型有所变化），可进行批量或连续操作。无荧光信号干扰，信噪比更高，并在筛选中提供卓越的 Z′ 值。

• 可定制或批量订购： 要了解更多关于此产品的定制信息，请浏览： www.promega.com/myway/

应用

• 测定病毒感染性及半数组织培养感染剂量 (TCID 50 )

• 测定化合物是否具有抗病毒效应

原厂资料：

The Viral ToxGlo™ Assay is a simple, quantifiable method of determining viral-induced cytopathic effects (CPE) in host cells caused by lytic virions. The assay measures cellular ATP as a surrogate measure of host cell viability. When CPE occurs due to viral infection, ATP depletion can be measured and correlated with viral burden. The amount of ATP detected is directly proportional to the number of viable host cells in culture and can be used as a simple method to quantify viral-induced CPE. The homogeneous "add-mix-measure" assay procedure involves adding the single reagent (ATP Detection Reagent) directly to host cells following viral treatment. A “glow-type” luminescent signal is generated that is proportional to the amount of ATP present. Cell washing, multiple pipetting steps and visual assessment are not required to assess CPE. The system detects as few as 15 cells/well in a 384-well format in 10 minutes after reagent addition and mixing and is designed for use in multiwell formats, making it ideal for automated high-throughput screening (HTS).

Features - Benefits

Objectively Quantify CPE: The assay provides quantifiable data by luminescence detection, which obviates subjective operator error associated with visual scoring methods.
Decrease Time to Results: Data can be recorded and analysis begun 10 minutes after reagent addition.
Simplify Assessment of CPE: The homogeneous “add-mix-measure” protocol dramatically reduces the manual steps required for CPE assessment.
Choose Your Format: The reagent is scalable from 96- to 1536-well plate formats.
Amenable to High Throughput Screening: Luminescent signal is very stable with a half-life generally >5 hours dependent on cell type and medium used, allowing batch or consecutive processing. No fluorescence interference results in high signal to background and delivers excellent Z′ values in screening applications.
Choose Your Reagent Configuration: Learn more about our custom options for this product at: www.promega.com/custom/

Applications

Determine viral infectivity and the corresponding tissue culture infective dose (TCID50).
Determine potential antiviral potency or off-target toxicity of test compounds.

Notes

This method is only useful for viruses that produce cytotoxicity and CPE.
Using 100μl of ATP Detection Reagent per assay in a 96-well format, Cat.# G8941 is sufficient to perform 100 assays; Cat.# G8942 and G8943, 1,000 assays. Using 25μl of ATP Detection Reagent per assay in a 384-well format, Cat.# G8941 is sufficient to perform 400 assays; Cat.# G8942 and G8943, 4,000 assays.