CellTox™ Green Dye, 1,000X【子货号：G873A，包装：5 x 20μl，，运保温度：-20℃】
描述：
说明
CellTox™ Green Cytotoxicity Assay(CellTox™ Green 细胞毒性检测试剂盒 ) 检测由于细胞死亡引起的细胞膜完整性变化。该试剂盒用于检测和评估实验处理对培养细胞的细胞毒性。 该试剂盒采用一种专利的非对称花青荧光染料 - CellTox™ Green Dye ，这种染料不能进入活细胞，但可以进入死细胞， 优先结合死细胞中的 DNA 。当染料结合从细胞中释放的 DNA 后，其荧光性能大幅提升，而活细胞产生的荧光没有明显的增加。因此，与死细胞 DNA 结合所产生的荧光信号与细胞毒性成正比。 CellTox™ Green Dye 对细胞无毒性作用，且处理细胞 72 小时后，荧光信号仍保持稳定，所以可用来检测长时间药物处理期间细胞毒性作用，或 终点法检测长时间处理后的细胞毒性作用。
特点
• 精确的细胞毒性测定： 72 小时药物处理后， CellTox™ Green Dye 仍可与 失去细胞膜完整性细胞中的 DNA 稳定结合，因此不会低估细胞毒性作用。
The CellTox™ Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation. The assay system uses a proprietary asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA from dead cells. When the dye binds DNA released from cells, its fluorescence properties are substantially enhanced. Viable cells produce no appreciable increases in fluorescence. Therefore, the fluorescence signal produced by the binding interaction with dead cell DNA is proportional to cytotoxicity. The CellTox™ Green Dye is non-toxic to cells, and the signal remains constant after exposure of 72 hours, making it ideal for determining toxic effects of treatments throughout an extended exposure or as an endpoint determination.
Features - Benefits
Accurate Cytotoxicity Determination: The CellTox™ Green Dye stably binds DNA of cells that have lost membrane integrity throughout 72-hour exposure and won’t underestimate cytotoxicity.
Kinetic Cytotoxicity Measures: Measure cytotoxicity at convenient time points from the same sample well to detect onset of toxicity with no duplication of plates.
Simple and Flexible Protocols: Add assay reagent directly to cells prior to plating or with dosing media to perform kinetic cytotoxicity measurements, eliminating a reagent dispensing step, or add diluted dye directly to cell culture wells as an endpoint add-mix-measure assay.
Multiplexing-Compatible: Get more informative data per well and reduce cell culture expenses by multiplexing with fluorescent and luminescent cell-based assays in the same well with no sample manipulation.
Easily Automated: Easily scale from 96- to 1536-well plate formats with “no-addition” or “single-addition” protocols.
Applications
Determine cytotoxic effects of treatments on cells in culture after long-term exposure.
References
McDougal, M. et al. (2010) Nucleic acid binding dyes and uses thereof. United States Patent Application 2010/0233710 A1.
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