The CellTiter-Blue® Cell Viability Assay provides a homogeneous, fluorescent method for monitoring cell viability. The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells rapidly lose metabolic capacity and thus do not generate a fluorescent signal. The homogeneous assay procedure involves adding the single reagent directly to cells cultured in serum-supplemented medium. After an incubation step, data are recorded using either a plate-reading fluorometer (preferred) or spectrophotometer.
Features - Benefits
Save Time:The homogeneous, add-incubate-measure format reduces the number of handling steps.
Perform More Than One Assay on the Same Sample:The system can be multiplexed with other assay methods such as the Apo-ONE® Homogeneous Caspase-3/7 Assay (Cat.# G7790) or the Caspase-Glo® Assays (Cat.# G8090, G8200, G8210) for detecting apoptosis.
Gain Flexibility:The CellTiter-Blue® Assay has an excellent Z´ factor and offers more flexibility in assay incubation times compared to other resazurin-based assays.
Safe:The reagent is generally nontoxic to cells, allowing extended incubation periods in some situations. Requires no scintillation cocktail, radioactive waste disposal (unlike [3H]-thymidine incorporation assays) or hazardous solvents (as required for MTT tetrazolium-based assays).
Adapt to Your Throughput Needs:The reagent is designed to provide sufficient volumes for accurate pipetting into 96- or 384-well formats. Convenient product sizes available for high-throughput screening.
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