将原包装中的整个系统避光储存于-20℃。一经融化,将系统组分( 除了GDNF Standard 即GDNF 标准品外) 储存于4℃。
原厂资料:
The GDNF Emax® ImmunoAssay Systems provide optimized reagents and a protocol for the sensitive and specific detection of glial cell-line-derived neurotrophic factor (GDNF). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound neurotrophic factor. Using this system, GDNF in tissue culture supernatants or tissue homogenates can be quantitated in the range of 15.6–1,000pg/ml.
Features - Benefits
High Value:Optimized reagents and protocol provided.
Specificity:Specific detection of GDNF; less than 3% cross-reactivity with other related neurotrophic and growth factors.
Sensitivity:Detect picogram levels of factor per milliliter of sample.
Flexibility: Available kits provide sufficient reagents for two or five 96-well plates; you can configure your plates as desired.
Plates are not included.
References
Okragly, A.J. and Haak-Frendscho, M. (1997)Exp. Neurol.145, 592–6.