The TGFβ1Emax® ImmunoAssay System provides optimized reagents and a protocol for the sensitive and specific detection of transforming growth factor β1(TGFβ1). After an overnight coating of a 96-well plate, the specific protein is detected using an antibody sandwich format. The system uses horseradish peroxidase-conjugated secondary antibody and a single-component TMB substrate for the final chromogenic detection of bound TGFβ1. Using this system, biologically active TGFβ1in tissue culture supernatants, plasma, serum or urine can be quantitated in the range of 15.6–1,000pg/ml.
Features - Benefits
High Value:Optimized reagents and protocol provided.
Specificity:Specific detection of TGFβ1; less than 3% cross-reactivity with other related growth factors (TGFβ2and TGFβ3).
Sensitivity:Detect picogram levels of factor per milliliter of sample.
Flexibility: Available kits provide sufficient reagents for two or five 96-well plates; you can configure your plates as desired.