Magne? Protein G Beads, 20% Slurry

产品编号：G7472 品牌：promega 原厂货号：G7472
产品分类：生化和细胞分析 > 微珠和磁珠 > 配体耦联磁珠 > Protein A&G 磁珠
应用分类：

包装：

5ml (5 × 1ml)

运保温度:

4°C

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其它组分：

Magne™ Protein G Beads【子货号：G747A，包装：5 x 1ml，，运保温度：4°C】

描述：

说明

Magne™ Protein G Beads 是从细胞培养基，腹水和血清标本纯化免疫球蛋白（ IgG ）的高特异性和高容量亲和磁珠。这些顺磁性磁珠由具有低非特异性结合能力的大孔纤维素外壳包裹铁内核组成。采用了一种新的附着原理将重组蛋白 G 蛋白分子以同一方向固定在磁珠表面。该磁珠提供了从多种生物样品中获得高纯度、高回收率的单克隆抗体和多克隆抗体的简便方法。 Magne™ Protein G Beads 拥有极佳的磁力特性，使得无论是手动处理的样品或采用自动化操作平台的高通量方式，如贝克曼 Biomek ® FX 或 Promega ReliaPrep™ LV32 HSM 仪器，均能快速、高效地捕获抗体。

特点

• 高容量： 每毫升磁珠可轻松纯化 >18mg 人类 IgG 。

• 易于处理： 最大限度地减少在纯化过程中的损失，磁珠独特的磁力特性可以增加样品通量。

• 高纯度 : 非特异性结合极低，以确保高质量的纯化。

• 优化的性能： 经验证可纯化从小体积（ 20µl ）到中等体积（ 30ml ）的样品。

应用

• 从血清、腹水和细胞介质样品中纯化抗体。

• 自动化、高通量纯化抗体。

• 小体积或稀释过的原料中纯化和浓缩抗体。

• 从血清或其他样品类型中免疫耗竭。

原厂资料：

Magne™ Protein G and Magne™ Protein A Beads are magnetic affinity beads with high specificity and high capacity for purification of immunoglobulins from cell culture media, ascites and serum samples. These paramagnetic beads are composed of iron encapsulated in macroporous cellulose with low nonspecific binding. The magnetic beads use a novel attachment chemistry to immobilize recombinant Protein G or Protein A protein molecules in the same orientation on the surface of the bead. The oriented attachment is known to improve the functionality of immobilized proteins. These beads offer a convenient method for achieving high purity and high recovery of monoclonal and polyclonal antibodies from a variety of biological samples. The superb magnetic properties of Magne™ Protein G and Magne™ Protein A Beads allow rapid and efficient capture of antibodies either with manually processed samples or in a high-throughput manner using the Promega ReliaPrep™ LV 32 HSM Instrument or a robotic platform such as the Beckman Coulter Biomek® FX.

Features - Benefits

High Capacity: Binding capacities in excess of 25mg per milliliter of settled beads are observed depending on antibody species and isotype.
Ease of Handling: Minimize losses during purification and increase sample throughput due to exceptional magnetic properties.
High Purity: Ensure high-quality purification because of low nonspecific binding on beads.
Optimized Performance: Use validated antibody purification methods for small (20µl) to medium (50ml) sample volumes.

Applications

Antibody purification from serum, ascites and cell-media samples.
Automated, high-throughput antibody purification.
Antibody purification and concentration from small-volume or dilute source material.

Notes

Magne™ Protein G and Magne™ Protein A Beads are not recommended for use in immunoprecipitation (IP) or co-IP applications.

注意事项：

不推荐将 Magne™ Protein G Beads 用于免疫沉淀（ IP ）或 co-IP 实验。

储存于 4°C 。不要冷冻。在储存或使用过程中不要让磁珠变干。