HaloTag? Mammalian Protein Detection and Purification System Sample Pack

• 产品编号：G6799      品牌：promega       原厂货号：G6799
• 产品分类：蛋白质表达与纯化 > 蛋白纯化 > 亲和填料和标准物
• 应用分类：

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原厂资料：

The HaloTag® Mammalian Protein Purification System (Cat.# G6790) is an optimized kit for purification of HaloTag® fusion proteins from mammalian cell culture lysates. HaloTag® fusion proteins form a highly specific and covalent bond with the HaloLink™ Resin. The covalent binding coupled with the low nonspecific binding of the HaloLink™ Resin provides superior purity and recovery of recombinant proteins from cultured mammalian cells, even at low expression levels. The HaloTag® Mammalian Protein Detection and Purification System (Cat.# G6795) also includes HaloTag® TMRDirect™ Ligand. The simple-to-use fluorescent detection of the HaloTag® fusion facilitates rapid optimization of expression and purification conditions.

Features - Benefits

• Purify More Protein: HaloLink™ Resin covalently binds >7mg/ml of HaloTag® fusion protein (10X more capacity compared to FLAG®). Recovery is highly efficient, commonly >75%.
• Higher Purity: Covalent capture allows extensive and/or stringent washes without loss of bound protein, resulting in very low (<0.1%) nonspecific binding and a highly pure protein.
• Easily Scalable: Scale up and down, important for obtaining mg-plus quantities.
• Optimized for Mammalian Protein Expression: The HaloTag® platform allows flexibility to move between purification, pull-downs and cellular imaging with a single construct.

Applications

• Purification of HaloTag® fusion proteins from mammalian cell culture lysates.

• 融合蛋白质的分离、纯化和检测