Blue/Orange 6X Loading Dye【子货号:G190A,包装:1 x 1ml,,运保温度:-20℃】
1kb DNA Ladder【子货号:G571A,包装:1 x 500μl,,运保温度:-20℃】
描述:
说明
DNA Ladders 是大小固定的ladder。该ladder 不作为定量分析使用。每一管Ladder 同时提供一管6× 蓝色/ 橙色上样染料。
1kb DNA Ladder:13 条平末端片段,大小范围250bp 到10,000bp。相对于其它片段,在溴化乙啶染色的琼脂糖凝胶上,亮度增强的1,000bp和3,000bp 片段易于辨认。其它片段具有相同的亮度。Ladder 经过脱磷酸处理,可直接使用T4 多聚核苷酸激酶进行放射性5` 末端标记,通过放射自显影观察。
特点
•推荐上样量:上样5μl/ 道。
储存条件
储存于-20℃。
原厂资料:
The DNA Ladders are ladders with defined sizes. The ladders are not intended for use in quantitative analysis. Each ladder is provided with a tube of 6X Blue/Orange Loading Dye.
1kb DNA Ladder: Thirteen blunt-ended fragments with sizes ranging from 250bp to 10,000bp. The 1,000bp and 3,000bp fragments have increased intensity relative to the other bands on ethidium bromide-stained agarose gels for easy identification. All other fragments are of equal intensity. The ladder is dephosphorylated and ready for 5´ end-labeling with radioisotopes using T4 Polynucleotide Kinase, allowing visualization by autoradiography.