CellTiter 96? AQueous Non-Radioactive Cell Proliferation Assay

产品编号：G5430 品牌：promega 原厂货号：G5430
产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞活性及活力检测试剂盒
应用分类：

包装：

5000 assays

运保温度:

-20℃，避光

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其它组分：

MTS Solution【子货号：G109C，包装：1 x 100ml，，运保温度：-20℃，避光】

PMS Solution【子货号：G110C，包装：1 x 5ml，，运保温度：-20℃，避光】

描述：

说明

CellTiter 96® AQ_ueous Non-radioactive Cell ProliferationAssay(CellTiter 96® AQ_ueous 非放射性细胞增殖检测试剂盒) 是一种均质、比色的方法，检测在细胞增殖、细胞毒性或化学敏感性分析中的活细胞数量。该试剂盒包含两种新型溶液，一种是四唑化合物[3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, 内盐；MTS]，另一种是电子耦合试剂PMS( 吩嗪硫酸甲酯)。MTS 可被生物还原为甲，后者可溶于组织培养基。甲在490nm 的吸光度可直接在96 孔板读出，而无需额外处理。MTS 到水溶性甲的转化由脱氢酶催化，而后者存在于有代谢活性的活细胞中。由490nm 处吸光度确定的甲的量直接与培养物中活细胞的数量成正比。

如果您目前正在使用[3H]- 胸腺嘧啶核苷掺入法，可使用MTS/PMS 混合溶液替代[3H]- 胸腺嘧啶核苷，在通常需加入[3H]- 胸腺嘧啶核苷时加入该试剂即可。以前的细胞增殖生物检测比较了[3H]- 胸腺嘧啶核苷掺入法与CellTiter 96® AQ_ueous 试剂盒的数据，并得到了相似的结果。这与使用以前的CellTiter 96®检测试剂盒进行类似的放射性掺入法研究是一致的。

CellTiter 96® AQ_ueous MTS Reagent Powder (CellTiter 96®AQ_ueousMTS 粉剂) 是一新型的四唑盐化合物，用于检测在细胞增殖、细胞毒性或化学敏感性分析中的活细胞数量，以粉剂的形式提供。

特点

• 易于使用：将MTS 和PMS 溶液混合，加入细胞培养体系，孵育，读取吸光度。

• 快速：在96 孔板里直接进行检测，无需洗涤或收集细胞。此外，该系统还省去了溶解甲产物的步骤，因为此试剂盒生成的甲产物在组织培养基内是可溶的。

• 非放射性：无需液闪混合液，也无需处理放射性废物( 如[3H]-胸腺嘧啶核苷)。

• 灵活：检测板可在读数后继续放回到培养箱中进一步显色( 与MTT 不同)。

• 安全：不需要使用挥发性的有机溶剂来溶解甲产物( 与MTT不同)。

原厂资料：

The CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay is a homogeneous, colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQueous Assay is composed of solutions of a novel tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS] and an electron coupling reagent (phenazine methosulfate) PMS. MTS is bioreduced by cells into a formazan product that is soluble in tissue culture medium. The absorbance of the formazan product at 490nm can be measured directly from 96-well assay plates without additional processing. The conversion of MTS into the aqueous soluble formazan product is accomplished by dehydrogenase enzymes found in metabolically active cells. The quantity of formazan product as measured by the amount of 490nm absorbance is directly proportional to the number of living cells in culture.
If you currently use a [3H]-thymidine incorporation assay, addition of the combined MTS/PMS solution can be substituted for [3H]-thymidine at the time point in the assay when the pulse of radioactive thymidine is usually added. Data from proliferation bioassays comparing the CellTiter 96® AQueous Assay and [3H]-thymidine incorporation show similar results. This is in agreement with similar radioactivity incorporation studies performed using the original CellTiter 96® Assay.

CellTiter 96® AQueous MTS Reagent Powder is a novel tetrazolium compound for use in colorimetric assays for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. It is provided in powdered form.

Features - Benefits

Easy to Use: Combine provided MTS and PMS solutions, add to cells, incubate and read absorbance.
Fast: Perform the assay in a 96-well plate with no washing or cell harvesting. Also eliminates solubilization steps because the MTS formazan product is soluble in tissue culture medium.
Non-Radioactive: Requires no scintillation cocktail or radioactive waste disposal (unlike [3H]-thymidine).
Flexible: Plates can be read and returned to incubator for further color development (unlike MTT).
Safe: Requires no volatile organic solvent to solubilize the formazan product (unlike MTT).

Applications

Cell proliferation.
Cytotoxicity.
Cell viability.
Chemosensitivity.
Cell attachment determinations.
Chemotaxis.

For more information, see the Protocols & Applications Guide.
References

Barltrop, J.A. et al. (1991) Bioorg. & Med. Chem. Lett. 1, 611.
Cory, A.H. et al. (1991) Cancer Commun. 3, 207–12.
Riss, T.L. and Moravec, R.A. (1993) Mol. Biol. Cell 3 (Suppl.), 184a.
CellTiter 96® Non-Radioactive Cell Proliferation Assay Technical Bulletin, #TB112, Promega Corporation.

注意事项：

要长期储存，请将MTS 和PMS 溶液储存于-20℃，避光保存。