Griess Reagent System

• 产品编号：G2930      品牌：promega       原厂货号：G2930
• 产品分类：生化和细胞分析 > 细胞分析试剂盒 > 细胞毒性检测试剂盒
• 应用分类：

描述：

Griess Reagent System(Griess 试剂系统) 检测亚硝酸盐(NO₂^-), 是两种主要的稳定和不挥发的一氧化氮 (NO) 的分解产物之一。一氧化氮是一种重要的生理功能信使和多种生物学系统的效应分子, 包括免疫、神经和心血管组织。此检测试剂盒依赖于Griess 在1879 年描述的重氮化反应，并经过了多年的改良。

Griess 试剂系统是基于在酸性条件下( 磷酸) 使用磺胺和NED(N-1-naphthylethylenediamine dihydrochloride) 的化学反应。此系统可对不同生理和实验的液体进行检测，如血浆、血清、尿液和组织培养基。亚硝酸盐灵敏度取决于基质。使用技术手册 #TB229 描述的操作程序，检测极限为2.5μM (125pmol) 亚硝酸盐( 在超纯的去离子蒸馏水中)。

原厂资料：

The Griess Reagent System measures nitrite (NO2–), which is one of two primary stable and nonvolatile breakdown products of nitric oxide (NO). Nitric oxide is an important physiological messenger and effector molecule in many biological systems, including immunological, neuronal and cardiovascular tissues. This assay relies on a diazotization reaction that was originally described by Griess in 1879. Through the years, many modifications to the original reaction have been described.

The Griess Reagent System is based on a chemical reaction that uses sulfanilamide and N-1-naphthylethylenediamine dihydrochloride (NED) under acidic (phosphoric acid) conditions. This system detects NO2– in a variety of biological and experimental liquid matrices such as plasma, serum, urine and tissue culture medium. The nitrite sensitivity is dependent on the matrix. The limit of detection is 2.5μM (125pmol) nitrite (in ultrapure, deionized, distilled water) using the protocol described in Technical Bulletin #TB229.

References

Bredt, D.S. and Snyder, S.H. (1994) Ann. Rev. Biochem. 63, 175–95.
Dawson, T.M. and Dawson, V.L. (1995) The Neuroscientist 1, 7–18.
Griess, P. (1879) Chem. Ber. 12, 426–8.

注意事项：

储存于 4℃。所有溶液都要以试剂盒自带的塑料瓶避光保存。