The HaloLink™ Resin provides a method for covalent and oriented attachment of HaloTag® fusion proteins onto a solid surface. The resin consists of a HaloTag® ligand bound to Sepharose® beads that specifically and rapidly binds HaloTag® fusion proteins. HaloLink™ Resin has high binding capacity. Due to covalent linkage, HaloTag® fusion proteins cannot be eluted from the resin, allowing extensive washing to remove nonspecifically bound protein without the danger of eluting HaloTag® fusion proteins. The binding rate is very rapid and equivalent to biotin-streptavidin.
The HaloLink™ Resin can be used in a variety of applications including: detection and analysis of protein:protein interactions (in vivo and in vitro), detection of enzymatic activity of immobilized HaloTag® fusions and one-step purification of fusion protein in conjunction with proteolytic cleavage. A variety of vectors for the expression of HaloTag® fusion proteins in bacterial, mammalian or cell-free systems are available. Please see the HaloTag® Technology Products page for more information.
The HaloTag® technology offers a quick and convenient way to test protein expression of HaloTag® fusion proteins as well as monitor the efficiency of immobilization to HaloLink™ Resin by labeling with fluorescent HaloTag® TMR Ligand followed by SDS-PAGE analysis. Guidelines can be found in the HaloLink™ Resin Technical Manual#TM250.
Features - Benefits
Covalent Attachment:Enables stringent washing, minimizing nonspecific background without dissociation of bound HaloTag® fusion proteins.
Fast Binding Kinetics:Enhances the detection of protein:protein interactions and enables binding of proteins at low concentrations.
Oriented Immobilization:Allows maximal enzyme activity of bound protein.
High Binding Capacity:One milliliter of settled resin binds >7mg of HaloTag® fusion proteins.
Applications
Covalent immobilization of HaloTag® fusion proteins.
Notes
Note:Size listed indicates amount of settled resin.
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