Pichia Protocols is the first all-inclusive collection of key procedures for use of the Pichia pastoris Expression System. Methods developed by the leaders in Pichia expression are provided as detailed, step-by-step protocols. This book will guide you from choosing the appropriate expression vector, through strain construction and analysis, to production of your protein of interest. The protocols in this book, part of the Methods in Molecular Biology Series, are written to permit any researcher familiar with molecular biology techniques to successfully use the powerful PichiaExpression System.
D. R. Higgins and J. Cregg, eds. The Humana Press, Totowa, NJ, 1998, 284 pp., hardcover.