FCSC815A is comprised of four coated populations and one blank population of uniform microspheres that are approximately the size of human lymphocytes (7-9µm). Each of the four sets is coated with goat-anti-mouse IgG (Fc-specific) with different calibrated Antibody Binding Capacities (ABC) for mouse monoclonal antibodies. The blank population has no specific binding capacity for mouse IgG.
When the bead populations are labeled in the same manner as the cells to be analyzed, they provide a means for constructing a QuickCal®calibration curve (ABC values vs. fluorescence intensity), from which samples may be "read". Cellular antigen expression may thus be quantitated in ABC units. The calibration curve is also a useful means for determining instrument linearity.
1 x 1ml bottle of uncoated microbeads
4 x 1ml bottles of coated microbeads
Buffer Solution
Borate buffer
Intended Use
FCSC815A is suitable for the quantitation of cellular antigen expression in Antibody Binding Capacity (ABC) units. This product may also serve as an accurate compensation standard when labeled with the same antibodies
Application
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visitwww.abdserotec.com/protocols.
Application Name
Yes
No
Not Determined
Suggested Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
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