Microspheres (also called latex beads or latex particles) are spherical particles in the colloidal size range that are formed from an amorphous polymer such as polystyrene. Our Molecular Probes® FluoSpheres® beads are manufactured using high-quality, ultraclean polystyrene and are loaded with a variety of our proprietary dyes to create intensely fluorescent beads that typically show little or no photobleaching, even when excited with the intense illumination required for fluorescence microscopy.
For tracking particles and cells, tracing fluid dynamics, discriminating the size dependence of uptake or transport in vivo, etc., we offer more concentrated, 0.04 µm diameter FluoSpheres® microspheres preparations (5% solids) that lack the sodium azide preservative present in the majority of our FluoSpheres® products. The 0.04 µm particles are small enough to be microinjected or to be taken up by phagocytosis.
We also offer 1.0 µm FluoSpheres® microsphere preparations that contain a much higher dye content than our other FluoSpheres® products. This allows stronger signals to be generated using fewer microspheres per tracing experiment.
In many biological systems, the concentrated fluorescence and spherical shape of the FluoSpheres® beads permit them to be detected against a relatively high but diffuse background fluorescence. Typically for these types of studies, the microspheres and the fluorescent dyes they contain are first extracted from the tissue sample, and then the fluorescence is quantified on a spectrofluorometer or fluorescence microplate reader. The use of fluorescent microspheres eliminates the radiation hazards and disposal hurdles associated with radiolabeled microspheres.
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For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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