FISH TAG DNA MULTICOLOR KIT W 1 KIT

产品编号：F32951 品牌：invitrogen 原厂货号：F32951
产品分类：DNA和RNA纯化 > 核酸分析 > CISH及FISH分析
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其它组分：

Alexa Fluor® reactive dyes【包装：5 vials ineach of 4pouches，，运保温度：≤–20˚C;desiccate;protect from light】

Dimethylsulfoxide (DMSO)【包装：200 μL，，运保温度：≤–20˚C;desiccate】

Dithiothreitol (DTT)【包装：100 μL，，运保温度：≤–20˚C】

Glycogen, ultrapure, nulcease free【包装：20 μL，，运保温度：≤–20˚C】

Sodium bicarbonate【包装：84 mg，，运保温度：≤–20˚C】

Water, nuclease free【包装：4.0 mL，，运保温度：≤–20˚C】

DNA nucleotide mix【包装：80 μL，，运保温度：≤–20˚C】

DNA polymerase I【包装：25 μL，，运保温度：≤–20˚C】

DNase I【包装：15 μL，，运保温度：≤–20˚C】

Nick translation buff er【包装：250 μL，，运保温度：≤–20˚C】

SlowFade® Gold antifade reagent【包装：2 mL，，运保温度：≤–20˚C; protect from light】

Binding buff er【包装：6.0 mL，，运保温度：≤25˚C】

Wash buff er【包装：3.2 mL，，运保温度：≤25˚C】

Elution buff er【包装：6.0 mL，，运保温度：≤25˚C】

Spin columns and collection tubes【包装：20 columnswith tubes，，运保温度：≤25˚C】

Collection tubes【包装：20 tubes，，运保温度：≤25˚C】

描述：

FISH Tag™ DNA kits provide a complete workflow solution for fluorescence in situ hybridization (FISH) applications. Each kit provides all of the reagents needed for probe synthesis, labeling, and purification, as well as for imaging the labeled specimen. FISH Tag™ DNA kits employ a two-step labeling technology, using nick translation to enzymatically incorporate an amine-modified nucleotide (aminoallyl dUTP) followed by chemical labeling with Alexa Fluor® dyes. [PureLink] nucleic acid purification technology is used for fast and efficient purification of the labeled probe, and SlowFade^® Gold antifade reagent for excellent photostability during imaging. FISH Tag™ DNA kits are ideal for labeling DNA targets in chromosomal spreads, and for in situ analysis of mitochondrial DNA localization and content. The FISH Tag™ DNA Multicolor Kit includes four spectrally distinct dyes - Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 594, and Alexa Fluor® 647 查看更多dyes - to allow simultaneous localization of multiple sequence-specific DNA targets. FISH Tag™ DNA kits are also available in single-color kits, each containing one of four Alexa Fluor® dyes.