Thermo Scientific Entranceposons are artificial DNA transposons. They are available as ready-to-use linear transposons or replicable plasmids. The plasmid Entranceposons allow the user to create transposons containing additional sequences of interest, e.g. marker genes. These custom transposons can then easily be inserted into the target DNA by taking advantage of the efficient Mu transposition reaction.
Entranceposons with wild type MuA recognition sites, without the polylinker
F-778 Entranceposon (CamR-3)
The marker gene cat confers resistance to chloramphenicol.
F-779 Entranceposon (KanR-3)
The marker gene npt confers resistance to kanamycin.
F-784 Entranceposon (TetR-3)
The marker gene tet confers resistance to tetracycline.
F-772 Entranceposon (supF-2)
See above.
F-774 Entranceposon (lacZ)
The marker gene lacZ codes for the -polypeptide of ß-galactosidase. Expression of the marker can be utilized for blue-white selection of the insertion clones.
F-775 Entranceposon (lacZ PT)
Promoter Trap Entranceposon for searching transcribed regions in target DNA. Expression of the promoterless marker gene lacZa requires transcription from a promoter in the target DNA upstream from the insertion site.
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