Thermo Scientific Entranceposons are artificial DNA transposons. They are available as ready-to-use linear transposons or replicable plasmids. The plasmid Entranceposons allow the user to create transposons containing additional sequences of interest, e.g. marker genes. These custom transposons can then easily be inserted into the target DNA by taking advantage of the efficient Mu transposition reaction.
Entranceposons with wild type MuA recognition sites, with a polylinker
F-764 Entranceposon (TetR)
The marker gene tet confers resistance to tetracycline.
F-771 Entranceposon (supF)
The marker gene supF codes for mutated tRNA that recognizes the amber stop codon (UAG) and can suppress a nonsense mutation in an E. coli strain. If the strain carries an amber nonsense mutation in a gene that is required for growth, for example an antibiotic resistance marker in a plasmid, the suppressing tRNA can insert tyrosine at the UAG stop codon, resulting in a full length gene product.
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