Replicator RNAi Kit

• 产品编号：F-610      品牌：Finnzymes       原厂货号：F-610
• 产品分类：Discontinued已停产
• 应用分类：

描述：

Perfectly duplexed RNA produced with Thermo Scientific Replicator RNAi Kit can be processed into siRNA, the key component in RNAi-mediated gene-silencing experiments. In the first step of the dsRNA synthesis, T7 DNA-dependent RNA Polymerase transcribes single-stranded RNA copies from the DNA template molecule.

Subsequently, the ssRNA molecules are directly converted to dsRNA by Phi6 RNA-dependent RNA polymerase in the same reaction mixture. One kit contains materials for producing up to 2.5 mg of pure double-stranded RNA in a 2 mL reaction volume. The high yield of double-stranded RNA obtained with the system is due to a unique combination of the complementary polymerase activities in carefully optimized reaction conditions.

The suitable DNA template is created by PCR amplification, which is carried out using the Thermo Scientific Phusion High-Fidelity DNA Polymerase, ensuring highest possible fidelity.

Highlights
No RNA hybridization step in the reaction protocol
RNA produced with this kit is perfectly duplexed
No smeared product resulting from RNA secondary structure formation or misannealing on agarose gels
Even large dsRNA fragments up to 20 kb, which are out of reach for competing systems, can be amplified

Applications
Production of double-stranded RNA in milligram quantities for gene silencing experiments based on the RNAi mechanism
Production of a dsRNA marker fragment with a specific size

Includes
Phusion High-Fidelity DNA Polymerase
5X Phusion HF Buffer, dNTP mix
λ Control DNA
Control Primer Mix
Phi6 Replicase
T7 RNA Polymerase
Inorganic Pyrophosphatase
10X dsRNA Synthesis Buffer
50 mM MnCl2 solution
10X NTP mix and 8 M LiCl solution

Production of dsRNA with the Replicator RNAi Kit

原厂资料：