产品组成： Phusion Site-Directed Mutagenesis Kit包含Phusion 热启动II DNA 聚合酶、5x Phusion HF Buffer、dNTP mix、Quick T4 DNA Ligase、2x Quick Ligation Buffer 以及带有control primer mix的对照质粒。
原厂资料：
Thermo Scientific Phusion Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions, or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with T4 DNA Ligase. The resulting plasmid can be then transformed into any competent E. coli cells.
Highlights
• Robust and reliable exponential amplification method
• No requirements, such as special vectors, restriction sites, or methylation status for the target plasmid
• No need to destroy the starting template in a separate step
•Phusion Hot Start II High Fidelity DNA Polymerase minimizes unwanted secondary mutations
• Amplification of large plasmids up to 10 kb
• Hot start modification of the polymerase prevents amplification of non-specific products and unwanted degradation of primers prior to first cycle of PCR
• T4 DNA Ligase included in the kit; no purification steps before or after ligation
• Compatible with all strains of competent E. coli cells
Applications
• Generating point mutations, insertions, and deletions in plasmid DNA
Includes
• Phusion Hot Start II DNA Polymerase (2 U/µL)
• 5x Phusion HF Buffer
• dNTP mix
• T4 DNA Ligase
• 5x Rapid Ligation Buffer
• Control Plasmid and Control Primer Mix
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