Thermo Scientific DyNAmo SNP Genotyping Master Mix在实时PCR仪上使用终点法荧光检测,可为客户提供快速、高质量的SNP基因分型。通过使用一种正在申请专利的master mix配方可实现对SNP等位基因进行可靠且可重复的检测。该配方可提供增强的分辨率,即使是靶向最具挑战性的扩增子时也能得到理想的结果。DyNAmo SNP Genotyping Master Mix经过专门的优化,使其在与TaqMan SNP Genotyping Assays或定制的分析试剂一起使用时,可以提供更优良的性能。DyNAmo SNP Genotyping Master Mix可在大多数实时热循环仪上提供理想性能。
优点:
缩短获得实验结果的时间——正在申请专利的Master Mix配方提供快速的实验方案(低至50分钟)
获得准确的基因分型结果(低至0.5ng DNA)
独特的双色系统可以帮助样品加样过程
可为高通量的实验室提供稳定一致的性能——预移取的反应液在4℃可以稳定放置3天
应用:
疾病位点的分型
突变分析
关联研究
流行病学研究
即使面对具有挑战性的扩增片段,DyNAmo SNP Genotyping Master Mix也能提供出众的性能
将DyNAmo SNP Genotyping Master Mix的性能与专门开发用于基因分型实验方案的三种行业领先的master mix进行比较。接受测试的所有Master Mix对LAC2都能提供可接受的检出率,但是对于更具挑战性的扩增片段(NOTCH2),这四种mix中的两种未能成功地检出正确的基因型。
Reliable and reproducible detection of SNP alleles is achieved via a patent-pending master mix formulation that offers enhanced discrimination, even when targeting the most challenging amplicons. Thermo Scientific DyNAmo SNP Genotyping Master Mix has been optimized to offer superior performance when using TaqMan SNP Genotyping Assays or custom designed assays. DyNAmo SNP genotyping Master Mix provides optimal performance on most real-time thermal cyclers.
Highlights
• Improve your time to results – Patent pending mix formulation delivers fast protocol times (as low as 50 minutes)
• Achieve accurate genotyping results with as little as 0.5 ng DNA
• A unique two color system aids sample set up procedures
• Consistent performance for high throughput laboratories – Pre-pipetted reactions stable for 3 days at 4°C
Applications
• Typing of disease loci
• Mutation analysis
• Association studies
• Epidemiology studies
Includes
2X master mix with blue dye (contains hot start version of an engineered Tbr DNA polymerase, optimized PCR buffer, MgCl2, dNTP mix including dUTP), 40X Sample Buffer with yellow dye and 50X ROX passive reference dye.