DyNAmo cDNA Synthesis Kit

• 产品编号：F-470L      品牌：Finnzymes       原厂货号：F-470L
• 产品分类：克隆 > cDNA合成，cDNA文库和文库构建 > cDNA合成
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描述：

Thermo Scientific DyNAmo cDNA Synthesis Kit专为两步法定量逆转录PCR (qRT-PCR)中的cDNA合成而设计，通常使用的扩增子的长度约为100 bp。DyNAmo cDNA Synthesis Kit包含qPCR反应中cDNA合成所必须的所有试剂。各种类型的RNA均可作为逆转录的模板，包括总RNA、mRNA、病毒RNA，以及体外转录的RNA等。试剂盒包含随机引物和oligo(dT)15引物。除了上述两种引物以外，还可以使用基因特异性引物。试剂盒所用逆转录酶是M-MuLVRNase H+。

Thermo Scientific DyNAmo cDNA Synthesis Kit is intended for cDNA synthesis in two-step reverse transcription-quantitative PCR (RT-qPCR) applications, where amplicons are usually around 100 bp in length. This kit can be used in conjunction with any qPCR master mix.

The DyNAmo cDNA Synthesis Kit includes a M-MuLV RNase H+ reverse transcriptase and all other necessary reagents for cDNA synthesis prior to qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed RNA can be used as a template for reverse transcription. The kit includes both random primers and oligo(dT) primers as separate vials, offering users flexibility in their choice of priming strategy, including the option to use gene-specific primers. Reagents deliver the specified number of reactions to a final reaction volume of 20 µL.

Highlights
•Broad dynamic range – up to 1 µg of RNA
•Reliable results with different RNA sources – mRNA, total RNA, viral RNA, and in vitro transcribed RNA
•Flexible – all priming options for cDNA synthesis
•Convenient to use – Short protocol
• Optimized 2X mix for cDNA synthesis

Applications
• RT-qPCR

Includes
• M-MuLV RNase H+ reverse transcriptase (includes RNase Inhibitor)
• 2X RT buffer (includes dNTP mix and 10 mM MgCl2)
•Random hexamers (300 ng/µL)
• Oligo(dT)15 primer (100 ng/µl)

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