DyNAmo Flash Probe qPCR Kit

• 产品编号：F-455XL      品牌：Finnzymes       原厂货号：F-455XL
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qPCR
• 应用分类：

描述：

Thermo Scientific DyNAmo探针法快速qPCR试剂盒 是探针法快速qPCR的理想选择。优化的试剂和极短的循环时间可以提供最快的速度，从而提高样品的通量。在传统和快速的实时PCR仪上使用均可显著地节约时间。DyNAmo探针法快速qPCR试剂盒的性能基于高效的热启动Thermus brockianus DNA 聚合酶，在较宽的模板浓度范围内都有高灵敏度和出色的扩增效率，保证了结果的可重复性。DyNAmo 探针法快速qPCR试剂盒专门针对水解探针(例如TaqMan探针)进行了优化，能在模块式和毛细管式实时PCR仪上使用。

优点：
• 极快的操作方案：退火和延伸步骤合并，只需15秒
• 在较宽的模板浓度范围内均可进行特异和灵敏的检测
• 含dUTP，可使用UNG防止残留污染
• 便捷的2x master mix和优化的操作方案

应用：
• 快速qPCR
• 使用序列特异性探针的qPCR
• 使用序列特异性探针的RT-qPCR



在较宽的模板浓度范围内均可获得高扩增效率
用DyNAmo 探针法快速qPCR 试剂盒和MJ/Bio-Rad 公司的Opticon 2 对Salmonella Typhimurium InvA基因的118bp扩增片段进行扩增(火和延伸步骤合并15秒，总循环时间67分钟)。DyNAmo 探针法快速qPCR 试剂盒在较短的时间内获得了很高的扩增效率。

产品组成
2x master mix (包含热启动Tbr DNA聚合酶、优化的PCR缓冲液、MgCl₂和含dUTP的dNTP mix) 和50x ROX校正染料。

原厂资料：

Thermo Scientific DyNAmo Flash Probe qPCR Kit is the perfect choice for fast probe-based qPCR. The optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. Performance of the DyNAmo Flash Probe qPCR Kit is based on an efficient hot start Thermus brockianus DNA polymerase. Sensitivity and the excellent amplification efficiency across a wide range of template concentrations guarantee reproducible results.

DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillary-based realtime PCR instruments.

Highlights
• Extremely fast protocols: combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2X master mix and optimized protocols

Applications
• Fast qPCR
• qPCR using sequence-specific probes
• RT-qPCR using sequence-specific probes

Includes

• 2X master mix (contains hot start Tbr DNA polymerase, optimized PCR buffer, MgCl2, and dNTP mix including dUTP)
• 50X ROX passive reference dye.

When using RNA as starting material, we recommend Maxima First Strand cDNA Synthesis Kit to ensure high-quality results.