DyNAmo ColorFlash SYBR Green qPCR Kit

• 产品编号：F-416XL      品牌：Finnzymes       原厂货号：F-416XL
• 产品分类：PCR/RT-PCR/qRT-PCR > RT-PCR/RT-qPCR > qPCR
• 应用分类：

描述：

Thermo Scientific DyNAmo ColorFlash SYBR Green qPCR Kit与DyNAmo Flash SYBR Green qPCR Kit的性能相当。另外，该试剂盒结合了一种能够确保正确移液的创新性多重颜色体系。2x master mix含有一种蓝色染料，单独的样品缓冲液含有黄色染料。将这两种组分混合后得到的qPCR mix是绿色的。使用这种多重颜色体系可以很容易地监控master mix和样品的移取，显著降低加样期间移液错误的风险，尤其是当使用白色反应管时，这种优点尤其明显。这些染料不会影响qPCR检测的特异性或灵敏度。DyNAmo ColorFlash SYBR Green qPCR Kit在大多数实时热循环仪上都可提供理想性能。

优点
• 最大程度地减少了加样期间的移液错误
• 当使用白色反应管时尤其有效
• 由于退火和延伸步骤合并，只需15秒，因此操作时间非常短
• 可在较宽的模板浓度范围内进行特异和灵敏的检测
• 2x master mix中含dUTP，可以使用UNG防止残留污染

应用
• 快速qPCR
• 使用SYBR Green染料的qPCR
• 使用SYBR Green染料的RT-qPCR


蓝色+ 黄色 = 绿色
DyNAmo ColorFlash SYBR Green qPCR Kit含有带蓝色染料的2x master mix。黄色的40x样品缓冲液在该试剂盒中以单管形式提供。当移取样品时，可将该缓冲液与样品混合以提供视觉上的帮助。


用透明板和白色板协助加样
白色反应管特别适用于qPCR应用，因为白色管可比透明管提供更高的信号强度。然而，传统的无色反应组分在白色孔中几乎看不到，这使得反应设置变得更困难。DyNAmo ColorFlash SYBR Green qPCR Kit克服了这种困难，降低了移液错误的风险。在左图中，蓝色孔中只含有2x master mix。绿色表示含有黄色染料的样品DNA也已经加入到反应液中。

产品组成
含蓝色染料的2x master mix (包含修饰的热启动Tbr DNA聚合酶、SYBR Green I、优化的PCR缓冲液、MgCl₂和含dUTP的dNTP mix)、含黄色染料的40x 样品缓冲液溶液以及 50x ROX 校正染料。

原厂资料：

Thermo Scientific DyNAmo ColorFlash SYBR Green qPCR Kit offers equal performance to the DyNAmo Flash SYBR Green qPCR Kit. In addition, it incorporates an innovative multi-color system that ensures correct pipetting. The 2X master mix contains a blue dye, and a separate sample buffer contains a yellow dye. The qPCR reaction mix containing both components is green. Using this patent-pending multicolor system, pipetting of both the master mix and the sample can be easily monitored. This significantly decreases the risk for pipetting errors during reaction setup, especially when using white reaction vessels. The dyes do not affect the specificity or sensitivity of qPCR assays.

Highlights
•Colored reagents minimize the risk of pipetting errors during reaction set up
• Especially beneficial when using white reaction vessels
•Extremely fast protocols due to combined annealing and extension step of only 15 s
•Specific and sensitive detection of a wide range of template concentrations
• dUTP included in the 2x master mix allows the use of UNG for prevention of carry-over contamination

Applications
• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye

Includes
• 2X master mix with blue dye (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, MgCl2, dNTP mix including dUTP)
• 40X sample buffer solution with yellow dye
• 50X ROX passive reference dye

Shipping and storage

DyNAmo ColorFlash qPCR Kit is shipped on gel ice. Upon arrival, store all kit components at -20°C. When using the 2X master mix, the leftover thawed mix can be refrozen and stored at -20°C without affecting the performance of the kit.

The yellow sample buffer solution is stable and can be stored at +4°C, but storage at -20°C with the other kit components is recommended.