Thermo Scientific DyNAmo Flash SYBR Green qPCR Kit 用于快速实时qPCR。在保证qPCR性能的前提下，该试剂盒比大多数SYBR Green试剂盒更快地提供qPCR结果。试剂盒中的DNA聚合酶上融合了一个DNA结合蛋白，可提高聚合酶-DNA复合物的稳定性，从而使扩增更持续、更强劲。DyNAmo Flash SYBR Green qPCR Kit 的高扩增效率能提供可靠的定量检测和更早的Cq值。由于具有高信号强度，DyNAmo Flash SYBR Green qPCR可提供出色的信噪比。DyNAmo Flash SYBR Green qPCR Kit在大多数qPCR仪上都可提供理想的性能。
应用
• 快速qPCR
• 使用SYBR Green 染料的qPCR
• 使用SYBR Green 染料的RT-qPCR
高效的扩增和高信号强度
用DyNAmo Flash SYBR Green qPCR Kit和MJ/Bio-Rad的Opticon 2 对人calmodulin基因的141bp的cDNA扩增子进行扩增(退火和延伸步骤合并15秒，总循环时间70分钟)。DyNAmo Flash SYBR Green qPCR Kit可得到高效的扩增和高信号强度。
产品组成 2x master mix (包含修饰的热启动Tbr DNA聚合酶、SYBR Green I、优化的PCR缓冲液、MgCl2和含dUTP的dNTP mix)以及50x ROX校正染料。
原厂资料：
DyNAmo Flash SYBR Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than most SYBR Green kits without compromising the qPCR performance. A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYBR Green qPCR Kit gives reliable quantification and early Cq values. Due to the high signal intensity, DyNAmo Flash SYBR Green qPCR provides an excellent signal-to-noise ratio.
Highlights
• Extremely fast protocols: Combined annealing and extension step of only 15 s
• Specific and sensitive detection of a wide range of template concentrations
• Included dUTP allows the use of UNG for prevention of carry-over contamination
• Convenient 2× master mix and optimized protocol
Applications
• Fast qPCR
• qPCR using SYBR Green dye
• RT-qPCR using SYBR Green dye
Includes
• 2X master mix (contains hot start version of a modified Tbr DNA polymerase, SYBR Green I, optimized PCR buffer, MgCl2, dNTP mix including dUTP)
• 50X ROX passive reference dye
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