Phire Animal Tissue Direct PCR

• 产品编号：F-140      品牌：Finnzymes       原厂货号：F-140
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > Direct PCR
• 应用分类：

其它组分：

描述：

 Phire动物组织直接PCR试剂盒用于直接从动物组织中扩增DNA，这些动物包括小鼠、鱼类、鸟类和昆虫等。试剂盒包含了一整套的优化试剂、制样工具和Thermo Scientific DNARelease添加剂。DNARelease能促进动物组织释放DNA。另外，试剂盒还包含详细的使用说明和用于不同物种的对照引物。

用Harris Uni-Core取样器和切片垫可以轻松获得大小均一的样本，然后按压取样器尾部活塞，将扎取的样本放入P C R 反应液（或稀释缓冲液）中。

优势：
● 无需进行费时而昂贵的DNA抽提
● 样品需求量小
● 两种简便的实验方案，适用于多种需求
● 试剂盒提供制样工具，便于操作
● Phire热启动II DNA聚合酶特异性好、产量高

测试过的样本举例：
● 小鼠组织（如：耳朵、尾巴、肝脏、脾脏、脑）
● 培养的小鼠细胞（成纤维细胞）
● 果蝇（翅膀、整体）
● 斑马鱼（鱼鳍）
● 线虫（整体）
● 狗（毛）
● 西伯利亚鸟类（羽毛）


从各种动物组织开始直接PCR（图1）
来自各种动物组织的少量样本直接置于50μl PCR反应液中。可用试剂盒内的对照引物进行特异DNA片段的扩
增，如果样本是果蝇和斑马鱼，可使用我们网站上提供的序列合成对照引物。
+和-代表含有纯化DNA模板和未含纯化DNA模板的对照。


检测F2转基因小鼠基因型（图2）
小鼠耳朵的0.50mm打孔组织直接置于50μl PCR反应液中，检测11只小鼠的基因型，每个反应使用2组引物。较
大的片段为490bp（转基因），较小的片段为250bp（野生型）。

原厂资料：

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.The kit contains all the necessary components for amplification of DNA directly from animal tissues: optimized reagents, sampling tools, and DNARelease Additive, which can be used to improve the release of DNA from animal tissues. In addition, the kit includes detailed instructions and control primers that can be used with numerous animal species.

Animal Direct PCR Protocol

Highlights
•No need for time-consuming and expensive DNA purification steps
•Very little sample material required
•Two simple protocols for various applications
•Convenient sampling tools included in the kit
•Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/kb).

Includes
•Phire Hot Start II DNA Polymerase
•2X Phire Animal Tissue PCR Buffer (includes dNTPs and MgCl2)
•Universal control primer mix
•Dilution Buffer
•DNARelease Additive
•Gel loading dye
•Harris Uni-Core™ 0.50mm
•Harris Cutting Mat™

Two alternative protocols suitable for various purposes
In the direct protocol, a small piece of animal tissue is placed directly into the PCR reaction. In the dilution protocol, a small amount of animal tissue is first briefly incubated in incubation buffer and 1 μL of this buffer is then added into the PCR reaction. The dilution protocol is useful if several PCR reactions are performed from the same sample.