Phire Plant Direct PCR Kit

• 产品编号：F-130      品牌：Finnzymes       原厂货号：F-130
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > Direct PCR
• 应用分类：

其它组分：

描述：

 Phire植物组织直接PCR试剂盒用于直接从植物样本中扩增DNA。在PCR扩增之前，无需进行DNA纯化。试剂盒包含一整套的优化试剂，制样工具和对照引物，使其成为扩增植物DNA的简便选择。

用Harris Uni-Core取样器和切片垫可以轻松获得大小均一的样本，然后按压取样器尾部活塞，将扎取的样本放入P C R 反应液（或稀释缓冲液）中。

优势：
● 无需进行费时而昂贵的DNA抽提
● 样品需求量小
● 两种简便的实验方案，适用于多种需求
● 试剂盒提供制样工具，便于操作
● Phire热启动II DNA聚合酶特异性好、产量高

测试过的样本举例：
叶片：
● 拟南芥
● 玉米、水稻、棉花
● 烟草、葡萄藤
贮存在滤纸上的样本：
● 玉米、番茄、豌豆
种子：
● 苹果、胡萝卜、南瓜
真菌和藓类：
● 稻瘟菌
● 菌根（杉木根部多个种类）
● 小立碗藓



叶片（图1）
将来自不同叶片的直径为0.50mm的小圆片直接置于50μl PCR反应体系。使用Phire植物组织
直接PCR试剂盒成功扩增多种来自于线粒体或基因组DNA的扩增子。
(+和-代表含有纯化DNA模板和未含纯化DNA模板的对照)
1 Arabidopsis, mtDNA (1 kb)
2 Arabidopsis, gDNA (3.5 kb)
3 Capsicum, mtDNA (1.4 kb)
4 Maize, mtDNA (1.4 kb)


种子（图2）
各类种子的小量样本以解剖刀切割后直接置于20μl PCR反应体系。1.3-1.5kb（根据不同种属）的
线粒体DNA片段被成功扩增。
(+和-代表含有纯化DNA模板和未含纯化DNA模板的对照)

原厂资料：

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR. The kit is based on Phire Hot Start II DNA Polymerase, a specially engineered enzyme with a DNA binding domain. The unique features of this DNA polymerase make it extremely robust and tolerant of many PCR inhibitors present in plant material.

Phire Plant Direct PCR Kit has been tested with leaves and seeds from a wide variety of plant species. The kit includes a complete set of optimized reagents, sampling tools and detailed protocols making it an ideal choice for amplification of plant DNA.When combined with Piko Thermal Cycler and Piko UTW PCR Plates, PCR protocols can be completed in as little as 30 minutes.

Highlights
•No need for time-consuming and expensive DNA purification steps
•Very little sample material required
•Two simple protocols for various applications
•Convenient sampling tools included in the kit
•Phire Hot Start II DNA Polymerase provides high yields of specific product with short extension time (20 s/ kb).

Applications
•Genotyping
•Transgene detection

Includes
•Phire Hot Start II DNA Polymerase
•2X Phire Plant PCR Buffer (includes dNTPs and MgCl2)
•Control Primer Mix (25 µM each)
•Dilution Buffer
•Harris Uni-Core™ 0.50 mm
•Harris Cutting Mat™

Two short Protocols for various needs
In the direct protocol, a small piece of plant material is placed directly in the PCR reaction mix. In the dilution protocol, a small volume of the Dilution Buffer containing crushed plant material is added in the PCR reaction mix. The dilution protocol is used for long or difficult amplicons, and for assays where several DNA fragments are amplified from the same sample.