Phire Hot Start II DNA Polymerase

• 产品编号：F-122L      品牌：Finnzymes       原厂货号：F-122L
• 产品分类：PCR/RT-PCR/qRT-PCR > PCR > 热启动PCR
• 应用分类：

描述：

Phire Hot Start II DNA Polymerase是一种可用于常规和高通量PCR的新型DNA 聚合酶。其独有的双链DNA结合蛋白结构域使之与普通Taq酶相比，延伸时间更短，产量更高，保真性提高了2倍。而基于Affibody技术的热启动修饰使该聚合酶可在标准的热循环条件下实现零时间激活。优点：

优点
• 快速热启动：无需额外热激活步骤
• 快速：比热启动Taq酶快四倍
• 强劲：对抑制因素的高耐受力最大限度的降低了反应体系的优化
• 产量高：酶的高效率可获得大量目的产物
• 更长的PCR产物：与其他热启动Taq酶相比，可扩增更长的DNA片段

应用
• 热启动PCR
• 常规PCR
• 非-高保真PCR
• 快速PCR
• 高通量PCR



完整的PCR实验方案耗时少于传统反应的一半（图1）
将来自五个厂家的热启动DNA聚合酶分别用于扩增一段600bp的人类基因组DNA片段。与化学修饰或使用抗体热启动技术的Taq DNA聚合酶(供应商A-D)相比，使用Phire热启动II DNA聚合酶，PCR实验最多可以快四倍。


更短时间内获得超高产量（图2）
将来自五个厂家的热启动DNA聚合酶用于扩增1.5kb的人类cathepsin K基因。仅用29分钟，Phire热启动II DNA聚合酶即可扩增得到大量的目标PCR产物。相比之下，其他四个重要厂家提供的热启动Taq DNA聚合酶（A-D）不仅耗时长，而且产量偏低。

几种高性能酶对比：
 

1. 单酶： DNA聚合酶，缓冲液，DMSO和MgCl2
2. Master Mix：2X master mix
3. 试剂盒：所有必需的PCR反应成分包括对照模板和引物

原厂资料：

Thermo Scientific Phire Hot Start II DNA Polymerase is a novel PCR enzyme for routine and high throughput PCR applications. It outperforms every Taq-based hot start polymerase on the market. Phire Hot Start II DNA Polymerase is significantly faster, extremely robust, and also capable of amplifying long DNA fragments with high yields. These features are achieved through advanced protein engineering of the polymerase. It incorporates a unique double-stranded DNA binding domain which allows short extension times (10 to 15 s/kb), improves yields, and increases fidelity 2-fold compared to Taq DNA polymerase.

The hot start modification of Phire Hot Start II DNA Polymerase is based on the same Affibody inactivation technology utilized by Phusion Hot Start II DNA Polymerase. This technology increases the specificity of the PCR reaction with no additional time required for initial activation of the enzyme.

New Phire Green format is a combination of Phire Hot Start II DNA Polymerase and 5X Phire Green Buffer. The buffer includes a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with enzyme performance and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Highlights
•Quick hot start – No reactivation step
•Fast enzyme – Amplify four times faster than with hot start Taq
•Robust – Minimal reaction optimization due to high inhibitor tolerance
•High yields – Abundant products due to high efficiency
•Longer PCR products – Amplify significantly longer DNA fragments than with any hot start Taq
•Direct loading on gel with Green Buffer

Applications
• Hot Start PCR
• Routine PCR
• Non-high fidelity PCR
• Fast PCR
• High throughput PCR
• Genotyping

Includes

Phire Hot Start II DNA Polymerase
• Phire Hot Start II DNA Polymerase
• 5X Phire Reaction Buffer (provides 1.5 mM MgCl2 in the final 1X concentration)
• DMSO

Phire Green Hot Start II DNA Polymerase
• Phire Hot Start II DNA Polymerase
• 5X Phire Green Reaction Buffer (provides 1.5 mM MgCl2 in the final 1X concentration)
• DMSO