PIERCE RAT IFN GAMMA ELISA KIT
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包装: |
192 assays |
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其它组分:
Anti-Rat IFN gamma 96-well Strip Plate, 2 each
Lyophilized Recombinant Rat IFN gamma Standard, 4 vials
Sample Diluent, 2 x 12mL
Biotinylated Antibody Reagent, 2 x 12mL
30X Wash Buffer, 2 x 50mL
Streptavidin-HRP Concentrate, 2 x 75μL
HRP Dilution Buffer, 2 x 13mL
TMB Substrate Solution, 2 x 12mL
Stop Solution, 2 x 13mL
Adhesive Plate Covers, 12 each
描述:
Assay IFN-γ (rat interferon gamma) levels in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Rat IFN gamma ELISA Kits.
These rat IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Highlights:
Target: natural and recombinant rat interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 8 to 500pg/mL
Sensitivity: < 2pg/mL
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 2.5 hours
Sample Size: 50μL
Specificity: no significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, or TNFα, or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250pg/mL mouse IFNγ was equivalent to 258pg/mL rat IFNγ (4.1% cross-reactivity).
Includes:
Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Details:
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Rat IFNγ ELISA standard curve. An example standard curve for the Thermo Scientific Rat IFNγ ELISA Kit (Part No. ERIFNG), with error bars based on six replicates for each interferon gamma standard concentration. |
原厂资料:
Assay IFN-γ (rat interferon gamma) levels in rat serum, plasma (EDTA, heparin, sodium citrate), and culture supernatant samples with our Thermo Scientific Rat IFN gamma ELISA Kits.
These rat IFN-gamma ELISA kits use pre-titered, matched pairs of coating and detection antibodies to achieve sensitive and accurate measurement of interferon gamma (IFNγ) in samples at 50 per well. Also included are calibrated recombinant protein standard, streptavidin-HRP, TMB substrate solution and stop solution. The complete kits contain precoated 96-well strip plate(s) and lot-optimized reagents. All components undergo quality control testing to minimize lot-to-lot variability.
Highlights:
Target: natural and recombinant rat interferon gamma (IFNγ)
Format: colorimetric ELISA (TMB substrate) in clear 96-well strip plate
Measurement: absorbance at 450nm minus absorbance at 550nm
Assay Range: 8 to 500pg/mL
Sensitivity: < 2pg/mL
Sample Types: cell culture supernatant, serum, plasma (EDTA, sodium citrate and heparin)
Total Assay Time: 2.5 hours
Sample Size: 50μL
Specificity: no significant cross-reaction with rat IL-1α, IL-1β, IL-2, IL-4, IL-6, IL-10, MIP-1α, RANTES, or TNFα, or human or pig IFNγ. When preparations of NIH/NIBSC mouse IFNγ (lot Gg02-901-533) were evaluated in the Rat IFNγ ELISA Kit, 6250pg/mL mouse IFNγ was equivalent to 258pg/mL rat IFNγ (4.1% cross-reactivity).
Includes:
Complete kits contain pre-coated antibody-plates, detection antibodies, buffers, diluents, standards, and substrates.
Details:
 |
Rat IFNγ ELISA standard curve. An example standard curve for the Thermo Scientific Rat IFNγ ELISA Kit (Part No. ERIFNG), with error bars based on six replicates for each interferon gamma standard concentration. |