BSM DNA POLYMERASE, LARGE FRAG

产品编号：EP0691 品牌：invitrogenH 原厂货号：EP0691
产品分类：克隆 > 聚合酶 > DNA聚合酶
应用分类：

包装：

1,600U (8U/u)

运保温度:

-20°C

到货周期：

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其它组分：

酶保存在：10 mM Tris-HCl (pH 7.5), 50 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.15% (v/v) Triton X-100, 50% (v/v) 甘油。

10X Bsm Buffer：200 mM Tris-HCl (pH 8.8, 25°C), 100 mM KCl, 100 mM (NH4)2SO4, 20 mM MgSO4, 1% (v/v) Tween 20。

描述：

Thermo ScientificBsm DNA Polymerase, Large Fragment, is a portion of DNA polymerase of Bacillus smithii, which catalyzes 5'=>3' synthesis of DNA and lacks 5'→3' and 3'→5' exonuclease activities. Bsm DNA Polymerase, Large Fragment, has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications.

Not suitable for use in PCR.

Highlights

Thermophilic DNA polymerase with strong strand displacement activity

Applications

Isothermal DNA amplification by the method of:
- Loop-mediated isothermal amplification (LAMP)
- Whole genome amplification (WGA)
- Ramification amplification (RAM)
Random-primed DNA labeling
Labeling by fill-in 5'-overhangs of dsDNA

原厂资料：

Thermo ScientificBsm DNA Polymerase, Large Fragment, is a portion of DNA polymerase of Bacillus smithii, which catalyzes 5'=>3' synthesis of DNA and lacks 5'→3' and 3'→5' exonuclease activities. Bsm DNA Polymerase, Large Fragment, has a strong strand displacement activity and is active in a wide range of temperatures from 30°C to 63°C, with an optimum of activity at 60°. It is an enzyme with high functional similarity to Bst DNA Polymerase, Large Fragment, and can replace it in most applications.

Not suitable for use in PCR.

Highlights

Thermophilic DNA polymerase with strong strand displacement activity

Applications

Isothermal DNA amplification by the method of:
- Loop-mediated isothermal amplification (LAMP)
- Whole genome amplification (WGA)
- Ramification amplification (RAM)
Random-primed DNA labeling
Labeling by fill-in 5'-overhangs of dsDNA

注意事项：

Use of this enzyme in certain applications may be covered by patents and may require a license.