Isoelectric focusing (IEF) is an electrophoresis technique that separates proteins based on their isoelectric point (pI). The pI is the pH at which a protein has no net charge and does not move in an electric field. IEF gels effectively create a pH gradient so proteins separate according to their unique pI. Novex® Pre-Cast Vertical IEF Gels for pI determination are excellent for native applications using soluble proteins. They can readily detect minor changes in a protein due to deamination, phosphorylation, or glycosylation, and can resolve different proteins of similar size which cannot be resolved on standard SDS-PAGE gels. Pre-focusing is not required. Total run time is approximately 2.5 hours. Formulation: Novex® IEF Gels are 5% polyacrylamide and consist of high-purity acrylamide, bisacrylamide, TEMED, APS, ultrapure water, and 2% ampholytes. They do not contain denaturing reagents. Recommended Buffers: IEF is a sensitive technique which is affected by many factors. Our optimized pre-mixed IEF buffers reduce variability and help you obtain consistent results.
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