Description: Amylose Resin High Flow is a cross-linked affinity matrix used for the isolation of proteins fused to maltose-binding protein (MBP). This rigid matrix can be used in automated chromatography systems.
Column Hardware Pressure Limit: 0.5 MPa or 75 psi
Maximum recommended Flow Velocity: 300 cm/hour
For a 1.6 cm column diameter: 10 ml/minute
For a 2.5 cm column diameter: 25 ml/minute
Column Buffer:
20 mM Tris-HCl (pH 7.4)
0.2 M NaCl
1 mM EDTA
Optional:
1 mM DTT or10 mM b-mercaptoethanol