pGL4.76[hRluc/Hygro] Vector

• 产品编号：E6941      品牌：promega       原厂货号：E6941
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 报告基因载体
• 应用分类：

其它组分：

描述：

无启动子的Renilla 萤光素酶载体是用来鉴定控制基因转录的重要区域中可能的启动子元件；也可以在萤火虫萤光素酶载体作为实验用载体的双萤光报告基因的系统中，用来作为无启动子的对照载体。我们提供含有三种经过改造的不同萤火虫萤光素酶基因(hRluc,hRlucP 或hRlucCP) 的无启动子载体。hRluc 基因经过改造，去除了大部分隐藏的转录因子结合位点，并通过编码优化提高了在哺乳动物细胞中的表达效率。hRlucP、hRlucCP 和RapidResponse^™ 基因是在hRluc 基因的基础上增加了降解序列，从而可以调控hRluc 基因在细胞中的半衰期。RapidResponse^™ 基因对刺激的响应更快，但是以牺牲信号强度为代价。hRlucP 基因反应比hRluc2 基因快，信号强度也适合，而hRlucCP 基因反应更快但信号强度最弱。无启动子载体有含选择标签(hygromycin，潮霉素) 和不含选择标签的可供订购。

原厂资料：

The pGL4.76[hRluc/Hygro] Vector encodes the luciferase reporter gene hRluc (Renilla reniformis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene that has been codon optimized for mammalian expression. The pGL4.76[hRluc/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region that allows cloning of a promoter of choice.

注意事项：

储存于-20℃。