收藏本站
热门搜索: A3500  E1910  A1360  25200056  dmem  

IMPACT Kit

 
包装:
运保温度: -20°C
到货周期: 登录后查看
标准价: ¥客户可见
会员价: ¥客户可见
积分: 客户可见
 
登录之后可加入购物车购买,请您 
对比 收藏

运费与支付说明:

1.含干冰类产品有运费;

2. 必须现金付款或有信用额度的会员才可以直接发货,否则需要等待现金付款信息。

描述:

特性:

♦ Single-column purification without the use of proteases to remove the affinity tag

♦ Able to produce target protein without vector–derived amino acids

♦ Fusion to either C-terminus or N-terminus of target protein

♦ Isolation of proteins with or without an N-terminal methionine residue

♦ Ligation and labeling of recombinant proteins

♦ T7 promoter-driven system to achieve high levels of expression and tight transcriptional control in E. Coli

 

Description:
The IMPACT™ (Intein Mediated Purification with an Affinity Chitin-binding Tag) Kit utilizes engineered protein splicing elements (termed inteins) to purify recombinant proteins by a single column (1-3) (Figure 1). This kit distinguishes itself from other protein fusion systems by its ability to separate a recombinant protein from the affinity tag without the use of a protease.

 

Kit Components:
E. coli Strain ER2566
1, 4-Dithiothreitol (DTT)
Anti-Chitin Binding Domain Serum
Blue Loading Buffer
Blue Loading Buffer Pack
Chitin Beads
pMXB10 Control Plasmid
pTXB1 Vector
pTYB21 Vector

 


相关图片

参考文献

本产品可用于的实验

购此产品的人还购买了
最近浏览产品

京ICP备15036693号-2    京公网安备11010802025653    版权所有:北京逸优科技有限公司       0.04

 纠 错