Description: A rapid method for gene expression analysis, PURExpress® is a novel cell-free transcription/translation system reconstituted from the purified components necessary for E. coli translation. The relative nuclease-free and protease-free nature of the PURExpress platform preserves the integrity of DNA and RNA templates/ complexes and results in proteins that are free of modification and degradation. Transcription and translation are carried out in a one-step reaction, and require the mixing of only two tubes. With results available in a few hours, PURExpress saves valuable laboratory time and is ideal for high throughput technologies.
Materials Not Included:
General: 37°C incubator
Labeling: 35S-Methionine (>1000 Ci/mmol recommended, in vitro translation grade)
TCA Precipitation: TCA solutions (25%, 10%), 1 M NaOH, casamino acids, ethanol, glass fiber filters, vacuum filtration manifold
SDS-PAGE: Gels and running buffer, gel apparatus, power supply, gel dryer
Western Blotting: Transfer apparatus, membrane, antibodies and detection reagent
Purification: Ni-NTA Agarose, Amicon Ultra- 0.5 ml, Ultracel- 100K Membrane Centrifugal Filters
Kit Components: E. coli tRNA (25 μl) Amino Acid Mixture (25 μl) Control (DHFR) template (10 μl) Solution A (50 μl) Solution B (75 μl)
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