pGL4.14[luc2/Hygro] Vector

• 产品编号：E6691      品牌：promega       原厂货号：E6691
• 产品分类：克隆 > 克隆载体和克隆试剂盒 > 载体 > 报告基因载体
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描述：

无启动子的萤火虫萤光素酶载体主要是用来鉴定控制基因转录的重要区域中的可能的启动子元件。我们提供含有三种经过改造的不同萤火虫萤光素酶基因(luc2, luc2P 或luc2CP) 的无启动子载体。luc2 基因经过改造，去除了大部分隐藏的转录因子结合位点，并通过编码优化提高了在哺乳动物细胞中的表达效率。luc2P、luc2CP 和RapidResponse^™ 基因是在luc2 基因的基础上增加了降解序列，从而可以调控luc2 基因在细胞中的半衰期。RapidResponse^™ 基因对刺激的响应更快，但是以牺牲信号强度为代价。luc2P 基因( 半衰期为1 个小时) 反应比luc2 基因( 半衰期为3 个小时) 快，信号强度也适合，而luc2CP 基因( 半衰期为0.4 个小时) 反应更快但信号强度最弱。这些无启动子载体含有不同选择标签(hygromycin( 潮霉素)、neomycin ( 新霉素) 或puromycin( 嘌呤霉素)) 和不含选择标签的可供订购。

原厂资料：

The pGL4.14[luc2/Hygro] Vector encodes the luciferase reporter gene luc2 (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. This vector also contains a mammalian selectable marker for hygromycin resistance in which the number of transcription factor binding sites has been reduced and mammalian codon usage optimized. The pGL4 Vectors are engineered with fewer consensus regulatory sequences than the pGL3 Vectors and a synthetic reporter gene, which has been codon optimized for mammalian expression. The pGL4.14[luc2/Hygro] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice.

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