EnduRen™ Live Cell Substrate is a proprietary compound that generates Renilla luciferase luminescence from live cells under normal growth conditions. The substrate produces luminescence with high signal-to-background ratios (high signal readings with low autoluminescence) that is very stable, allowing luminescence measurement for at least 24 hours after substrate addition. EnduRen™ Substrate may be used in a variety of cell analysis techniques including reporter gene analysis, RNAi analysis and Bioluminescence Resonance Energy Transfer (BRET) analysis, because the substrate permits real-time measurement in multiwell plates.