The NEBNext® ChIP-Seq Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
Lot Control:
The lots provided in the NEBNext ChIP-Seq Library Prep Master Mix Set for Illumina are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
Functional Validation:
Each set of reagents is functionally validated together through construction and sequencing of a genomic DNA library on an Illumina Sequencer.
The Reagent Set Includes:
The volumes provided are sufficient for preparation of up to 10 reactions (NEB #E6240S) and 50 reactions (NEB #E6240L). (All reagents should be stored at -20°C):
♦ NEBNext End Repair Enzyme Mix
♦ NEBNext End Repair Reaction Buffer (10X)
♦ Klenow Fragment (3´→ 5´ exo–)
♦ Deoxynucleotide Solution Mix
♦ NEBNext dA-Tailing Reaction Buffer (10X)
♦ Quick T4 DNA Ligase
♦ NEBNext Quick Ligation Reaction Buffer (5X)
♦ Phusion High-Fidelity PCR Master Mix with HF Buffer*
* Phusion® DNA Polymerase was developed by Finnzymes Oy, now a part of Thermo Fisher Scientific. This product is manufactured by New England Biolabs, Inc. under agreement with, and under the performance specifications of Thermo Fisher Scientific.
Phusion® is a registered trademark and property of Thermo Fisher Scientific.
Illumina® is a registered trademark of Illumina, Inc.
SuperScript® is a registered trademark of Invitrogen Corporation.
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