NEBNext ChIP-Seq Library Prep Reagent Set for Illumina

产品编号：E6200S 品牌：New England Biolabs 原厂货号：E6200S
产品分类：DNA和RNA纯化 > 核酸分析 > 测序试剂和试剂盒 > 测序样本文库制备
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包装：

运保温度:

-20°C

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描述：

Description:
The NEBNext® ChIP-Seq Library Prep Reagent Set for Illumina® contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing of Chromatin Immunoprecipitated DNA. Each of these components must pass rigorous quality control standards and are Lot Controlled, both individually and as a set of reagents.

Lot Control:
The lots provided in the NEBNext ChIP-Seq Library Prep Reagent Set for Illumina are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.

Functionally Validated:
Each set of reagents is functionally validated together through construction and sequencing of a ChIP DNA library on an Illumina Sequencer.

The Reagent Set Includes:
The volumes provided are sufficient for preparation of up to 12 reactions (NEB #E6200S) and 60 reactions (NEB #E6200L). (All reagents should be stored at -20°C).

♦ Phosphorylation Reaction Buffer (10X)

♦ Deoxynucleotide Solution Mix (10 mM each dNTP)

♦ T4 DNA Polymerase

♦ DNA Polymerase I, Large (Klenow) Fragment

♦ T4 Polynucleotide Kinase

♦ Deoxyadenosine 5´- Triphosphate (dATP) (1.0 mM)

♦ Klenow Fragment (3´→5´ exo^–)

♦ NEBuffer 2 for Klenow Fragment (3´→5´ exo^–) (10X)

♦ Quick T4 DNA Ligase

♦ Quick Ligation Reaction Buffer (2X)

♦ Phusion High-Fidelity PCR Master Mix with HF Buffer (2X)*

♦ NEBNext High-Fidelity 2X PCR Master Mix (SAMPLE)