The NEBNext Small RNA Library Prep Set for SOLiD contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the SOLiD platform. NEBNext Small RNA Sample Prep Set 3 is multiplex compatible. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
Lot Control:
The lots provided in the NEBNext Small RNA Sample Prep Set 3 are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
Functionally Validated:
Each set of reagents is functionally validated together through construction and sequencing of a small RNA library on the SOLiD sequencing platform.
The Set Includes: The volumes provided are sufficient for preparation of up to 10 reactions (NEB #E6160S) and 50 reactions (NEB #E6160L). (All reagents should be stored at -20°C).
NEBNext® 3´ Ligation Reaction Buffer (2X)
NEBNext® 3´ Ligation Enzyme Mix
NEBNext® 3´ SR Adaptor 3
NEBNext® 5´ SR Adaptor 3
NEBNext® 5´ Ligation Reaction Buffer (10X)
NEBNext® 5´ Ligation Enzyme Mix
NEBNext® SR RT Primer 3
NEBNext® First Strand Synthesis Reaction Buffer
M-MuLV Reverse Transcriptase (R Nase H-)
Murine RNase Inhibitor
OneTaq Hot Start 2X Master Mix with Standard Buffer
NEBNext® Index I Primer for SOLiD
NEBNext® SR Primer R3
Gel Loading Dye, Blue (6X)
Quick-Load® pBR322 DNA-MspI Digest
DNA Gel Elution Buffer, 1X
Linear Acrylamide (10 mg/ml)
TE Buffer
Nuclease-free Water
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