The NEBNext Small RNA Library Prep Set for Illumina contains adaptors, primers, enzymes and buffers that are ideal to convert small RNA into cDNA libraries for next-generation sequencing on the Illumina® platform. Each of these components must pass rigorous quality control standards and are lot controlled, both individually and as a set of reagents.
Lot Control:
The lots provided in the NEBNext Small RNA Library Prep Set for Illumina are managed separately and are qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page.
Functionally Validated:
Each set of reagents is functionally validated together through construction and sequencing of a small RNA library on the Illumina sequencing platform.
The Set Includes: The volumes provided are sufficient for preparation of up to 10 reactions (NEB #E6120S) and 50 reactions (NEB #E6120L). (All reagents should be stored at -20°C).
♦ NEBNext® 3´ Ligation Reaction Buffer (2X)
♦ NEBNext® 3´ Ligation Enzyme Mix
♦ NEBNext® 3´ SR Adaptor 1
♦ NEBNext® 5´ SR Adaptor 1
♦ NEBNext® 5´ Ligation Reaction Buffer (10X)
♦ NEBNext® 5´ Ligation Enzyme Mix
♦ NEBNext® SR RT Primer 1
♦ Deoxynucleotide Solution Mix (10 mM each dNTP)
♦ Murine RNase Inhibitor
♦ LongAmp® Taq 2X Master Mix
♦ NEBNext® SR Primer F1
♦ NEBNext® SR Primer R1
♦ Gel Loading Dye, Blue (6X)
♦ Quick-Load Low Molecular Weight DNA Ladder
♦ DNA Gel Elution Buffer, 1X
♦ Linear Acrylamide (10 mg/ml)
♦ TE Buffer
♦ Nuclease-free Water
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